2_Lab 2_Materials-Methods_W2009

2_Lab 2_Materials-Methods_W2009 - Chemistry 150 K Marr...

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Chemistry 150 - K. Marr Revised Winter 2009 Green River Community College Page 1 of 2 Lab 2. Spectroscopic Determination of Allura Red:How Much Dye is in my Gatorade? Materials and Methods Materials - Stock solution of FD&C Red No. 40 (Allura Red) - Gatorade Fruit Punch (FD&C red dye #40) - Allura Red Unknown record number in your lab notebook! - Five (5) 100.0 mL Volumetric flask - Various pipettes - Pipette bulbs - Vernier spectrophotometer - Cuvettes - Kimwipes - D.I. Water I. Preparation of Standard Solutions of Allura Red from Stock Solution 1. Prepare the first of the six standard solutions by quantitatively pipetting 3.00 mL of the stock solution into a 100 mL volumetric flask . Dilute to the mark and mix well. Label the flask with your name and the number “3”. 2. Repeat this procedure using 5.00, 10.00, 15.00, 20.00 and 25.00 mL samples to prepare the remaining standard solutions. The standard solutions will be used to generate the calibration curve and to determine max . For convenience, later in the lab the standard solutions will be referred to as solution 3, solution 5, etc. Later, the exact concentration (in mol/L) of these stock solutions must be calculated. II. Determination of max with a “ Vernier Spectrop hotometer” 1. Use a USB cable to connect a spectrometer to the computer.
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