ch03 - Chapter 3 Exploring Proteins and Proteomes Matching...

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Unformatted text preview: Chapter 3 Exploring Proteins and Proteomes Matching Questions Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. a) HPLC b) specific activity c) MALDI-TOF mass spectrum d) zonal centrifugation e) nascent f) SDS g) epitope h) Svedberg i) immunoglobulin j) centrifugation k) overlap peptides l) affinity chromatography 1 The ratio of enzyme activity relative to total protein is called ____________. Ans: b Section: 3.1 2 The first step in protein purification from a homogenate is usually ____________. Ans: j Section: 3.1 3 ____________ A type of purification that is based on the attraction of the protein for a particular chemical group. Ans: l Section: 3.1 4 ____________ can be added prior to gel electrophoresis to denature the proteins. Ans: f Section: 3.1 5 Sedimentation coefficients are described as ____________ units. Ans: h Section: 3.1 6 Proteins with different sedimentation coefficients can be separated by ____________. Ans: d Chapter 3 Exploring Proteins and Proteomes Section: 3.1 7 In order to sequence a whole protein, ____________ are used. Ans: k Section: 3.2 8 ____________ The name used to describe the original, uncleaved protein. Ans: e Section: 3.2 9 ____________ Another name for an antibody. Ans: j Section: 3.3 10 ____________ Another name for an antigenic determinant. Ans: g Section: 3.3 Fill in the Blank Questions 11 Proteins can be separated from small molecules and ions through a semi-permeable membrane by __________________. Ans: dialysis Section: 3.2 12 Exclusion gel or gel-filtration chromatography separates molecules on the basis of __________________. Ans: size Section: 3.1 13 __________________ is a chemical reagent that is often used to detect the presence of amino acids. Ans: Ninhydrin Section: 3.2 14 In the Edman procedure for peptide sequence, phenyl isothiocyanate is used to selectively remove the __________________ residue as a PTH-derivative. Ans: N-terminal Section: 3.2 15 Disulfide bonds in peptides and proteins are readily oxidized to cysteic acid residues by treatment with __________________. Ans: performic acid Section: 3.2 16 MALDI-TOF is the abbreviation for __________________. Ans: Matrix-Assisted Laser Desorption and Ionization Time of Flight mass spectrometer Section: 3.5 2 Chapter 3 Exploring Proteins and Proteomes 17 Automated peptide synthesis involves the activation of the carboxyl group of the incoming amino acid by __________________ and then reaction with the amino group of the growing peptide chain....
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ch03 - Chapter 3 Exploring Proteins and Proteomes Matching...

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