CELL chemotaxis of dictyostelium under varying pH

CELL chemotaxis of dictyostelium under varying pH -...

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Unformatted text preview: CHEMOTAXIS OF DICTYOSTELIUM UNDER VARYING pH Michael Kjome University of Colorado at Boulder Cellular Biology, MCDB 3140 TA: Mitesh Shridhar October 29, 2008 Activity of Dicty in Varying Acid/Base Environments Abstract The activity of dictyostelium towards folic acid, its natural source of nourishment, will be observed under varying the internal pH of the dictyostelium cells. Dictyostelium move toward folic acid by a process known as chemotaxis. Chemotaxis is a method by which cells direct their movement toward certain chemicals in their environment. We hope to observe a change in the rate of chemotaxis by altering the internal pH of dictyostelium cells by immersing them in different acids and bases of known pH. The internal pH of the dictyostelium will be altered by the use of a proton pump inhibitor. This will hinder the cell from obtaining its natural internal pH. Allowing us, by soaking the cells, to determine the internal pH we chose to study. Introduction Dictyostelid are a group of slime molds or social amoeba which act as singular organisms normally, they feed and divide normally. When there is scarcity in their source of food they aggregate and form a multicultural assembly. The multicellular body acts as a whole and has a defined anterior and posterior end as well as responds to light and temperature gradients. Under some circumstances the slug matures a fruiting body, at the tip of the fruiting body there are inactive cells which will become new amoeba when food is present. Dictyostelium uses chemotaxis for its movement towards its food source by recognizing the pH gradient. The natural food source for dictyostelium is folic acid which has a pH around 7.8. When dictyostelium senses a pH of 7.8 the cell moves toward the gradient and feeds on the source. Folic acid in an aqueous solution it is very sensitive and decomposes rapidly. Meaning there is constant movement for Dicy to find sources of food and the process of chemotaxis is integral in dictyostelium survival. The importance chemotaxis plays in the survival of dictyostelium makes it a prime candidate for and experiment on chemotaxis. Method This experiment will be done on a two week period although the steps taken will take a full two weeks. For the first week we will perform a test experiment to determine which reagent we would like to look into and which would end up killing the cells. Since we have a limited knowledge of what pH will terminate Dicty this will show at which internal pH they can function. For the experiment we will begin with a sample of Dicty cells at 6 x 10^6 cells per mL. With the cells we will wash them three times in the KK2 cells at 6 x 10^6 cells per mL....
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CELL chemotaxis of dictyostelium under varying pH -...

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