Methylene Blue Lab Procedure

Methylene Blue Lab Procedure - 5. Measure the absorbance of...

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Marc Pomi 10640098 September 16, 2009 Professor Johnson and Oatis Methylene Blue Procedure: Part A: 1. Obtain 25 mL samples of 10 -4 M Methylene Blue solution in 3 Petri dishes. 2. To two of the samples, add 25 mg of TiO 2 . Immediately cover all samples and seal the outsides with tape to prevent evaporation. Place one of the two samples immediately in a bag to prevent light exposure. 3. Place the two uncovered samples in the sun for 60 minutes and measure light intensity with a Radiometer. 4. Filter each sample using filter paper with 0.22 µm mesh rating.
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Unformatted text preview: 5. Measure the absorbance of each sample at 660 nm using a spectrophotometer. Part B: 1. Obtain 25 mL samples of 10-4 M Methylene Blue solution in 7 Petri dishes. 2. Add 25 mg of TiO 2 to all Petri dishes and cover immediately and seal with tape. 3. Place all samples in sun light. 4. Immediately withdraw 1 sample and repeat steps 4 and 5 in Part A then, withdraw the remaining samples in 10 minute intervals....
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Methylene Blue Lab Procedure - 5. Measure the absorbance of...

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