ps 3 - moon [Gaol ma Chemistry of Food and Bioprocesscd...

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Unformatted text preview: moon [Gaol ma Chemistry of Food and Bioprocesscd Materials 402 FALL 2009 Problem set #3 20 pts Due on October 19, 2009 Attached are spectra for chlorophyll a, chlorophyll b and carotenoids. They are often foimd together in plants and we need to be able to determine the concentration of each pigment in the mixture. - 1. Each pigment has two or three major peaks. On the figure, label the peaks in each spectrum as l, 2 and possibly 3 starting from left to right. Since there are 3 spectra, there should be three sets of numbers. 2. Determine the wavelength for each of the three MAJOR peaks for each pigment and use that data to fill-in the following chart. Peak #1 Chloroh 11a "“f/O Chloro h 11 b . "4% Carotenoids 'r I o\’\ O 3. If you had a solution of just chlorophyll b, what wavelength would you use to determine your extinction coefficient? Based on that wavelength, and assuming that the data is for a 1 mM concentration of pigment and the path length is 1 cm, calculate the Molar Extinction Coefficient for chlorophyll b (note: to determine the precise absorbence you need to determine the scale of the y—oxis). 4. Answer the following questions about the article “Fish out of water” that was handed out in class. a. From a nutrition/health standpoint, why should we consume omega-3 fatty acids? b. What is the source for omega—3 fatty acids infish‘? c. From a bioprocessing standpoint, compare the different approaches that Dow AgroScience and Monsanto are taking to increase omega-3 fatty acids in the diet. d. Which approach is best [or food quality and why? Amount at tight absorbed Waveiength cf fight (um) Spectra fiom: ' 7 http://images. goo gle.com/imgres?imgur1=http ://www.uic.edu/classes/bios/bios 1 OO/lecturesf04am labsorption—spectrum.jpg&imgrefurl=http ://www.electIo—tech-online,com/electronic-proj ects— design-ideas—reviews/ 9563 2-led-light- 3 .htm1&usg=_1 Q94BXDKt1VzZGXJ7LRCfHWOh1 ?&h=601&w=801&sz=25&hl=en&start= 21 &um=1&tbnid=q1mHKj5KOOvAcM:&tbnh=l O7&tb11w=143&preF/images%3Fq%3Dcante noid%2Bspectra%26ndsp%3D21 %26h1%3Den%26client%3Dfirefox- a%26rls%3 Dorgmozilla: en—US :official%26sa° o3 DN%26um%3D1 ...
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This note was uploaded on 12/10/2009 for the course FS 402 taught by Professor Dr.e.allenfoegeding during the Spring '09 term at N.C. State.

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ps 3 - moon [Gaol ma Chemistry of Food and Bioprocesscd...

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