LW_BCH4122_Lecture6_2009Sep-1 edit

LW_BCH4122_Lecture6_2009Sep-1 edit - 1. Epigenetic...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
1. Epigenetic Reprogramming of Somatic Cells ES b. Cell fusion c. Cell extract d. Culture induced a. Nuclear/ chromosome transfer e. Genetic reprogramming Oct4+cMyc +Klf4+Sox2 (Cell 2006;126:677) Somatic cells (Nature 2006; 441: 1061-1067. Cell 2007; Science 2007; Nature 2008; Science 2008 …)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
a. Somatic cell nuclear transfer (SCNT) reprogramming ES, NSC, T, B cells, granulocytes, etc (Cell 2006;126:652) Unsuccessful for hESC
Background image of page 2
e. Genomic Reprogramming NATURE 2007;448:260
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
v Screening the most potential genes for reprogramming - In total, 24 genes previously implicated in the biology of ES cells were tested. These genes included some known to be involved in the process of self-renewal (Oct3/4, Sox2, and Nanog) and some more commonly associated with transformation that have also been implicated in the maintenance of ES cell pluripotency (c- Myc, Eras, and Klf4). v To induce reprogramming, the authors cotransduced the target fibroblasts with retroviral vectors, each carrying a unique cDNA encoding a candidate
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 7

LW_BCH4122_Lecture6_2009Sep-1 edit - 1. Epigenetic...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online