LW_BCH4122_Lecture7_2009Sep-1 edit

LW_BCH4122_Lecture7_2009Sep-1 edit - 1. Early development...

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Ectoderm v Central nervous system v Peripheral nervous system v Sensory epithelia of the eye, ear, and nose v Mammary glands v Pituitary gland v Subcutaneous glands v Enamel of teeth Endoderm v Liver v Pancreas v Thymus v Thyroid v Parathyroid glands v Epithelial lining of the gastrointestinal and respiratory tracts v Epithelial lining of the tympanic cavity v Epithelial lining of the urinary bladder and most of the urethra v Pharyngotympanic or auditory tube v Tonsils v Tympanic antrum v Blood v Bone v Cartilage v Heart v Blood vessels v Striated and smooth muscles v Lymphatic vessels v Spleen v Membranes of the body cavities (pericardium, pleura and peritoneum) v Kidneys v Genital ducts v Ovaries v Testis v Connective tissue v Cortex of the adrenal glands Mesoderm 1. Early development of human embryo - Tissues derived from three germ layers
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3. Maintenance (self-renewal) of human embryonic stem cells Maintenance (self-renewal) - signaling balance of proliferation , apoptosis, and differentiation Single cell analysis is essential for any definitive characterization of self-renewal.
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3. Maintenance (self-renewal) of hESCs 1. Feeder cell culture (very good) : mouse embryonic fibroblast cells or human feeder cells + bFGF + serum replacement 1. Non-feeder cell culture (good): •.
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LW_BCH4122_Lecture7_2009Sep-1 edit - 1. Early development...

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