LW_BCH4122_Lecture7_2009Sep-1

LW_BCH4122_Lecture7_ - Lecture 7 Human Embryonic Stem Cells(hESC Lisheng WANG Department of Biochemistry Microbiology and Immunology Faculty of

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 7: Human Embryonic Stem Cells (hESC) Lisheng WANG Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
1. Development of human embryo 2. Origin of hESCs 3. Maintenance of hESCs 4. Examination of undifferentiated hESCs 5. Differentiation of hESCs 6. Differences between human and mouse ESCs 7. Ethics of hESCs 8. Application of hESCs Human embryonic stem cells
Background image of page 2
Embryonic stem (ES) cells: milestones and achievements 1981 : The first isolation and establishment in culture of pluripotent mouse ES cells 1988: Identification of leukemia inhibitory factor (LIF) as being crucial for maintaining the pluripotency of mouse ES cells in vitro 1996: Successful somatic cell nuclear transfer (SCNT) responsible for cloning of the first mammalian species 1998: Genetic selection of pure populations of terminally differentiated cell types from mouse ES cells 1998 : The first derivation of human ES cells 2000: Generation of nuclear transfer ES cell lines by SCNT in mice 2001: The first report - genetic modification of human ES cell lines 2002: Generation of histocompatible tissues by SCNT in cattle 2002: Correction of a congenital defect in mice by combining genetic manipulation of nuclear transfer ES cell lines 2002: Treatment of an animal model of Parkinson’s disease using dopaminergic neurons differentiated from ES cells. 2007 : Induced pluripotent stem cells (iPS) from human fibroblast cells (Paul J. Fairchild 2004; Science 2007; Cell 2007)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
1. Early development of human embryo -From oocyte to three germ layers Blastocyst Endoderm (Internal layer) Mesoderm (middle layer) Ectoderm (external layer)
Background image of page 4
Ectoderm Central nervous system Peripheral nervous system Sensory epithelia of the eye, ear, and nose Mammary glands Pituitary gland Subcutaneous glands Enamel of teeth Endoderm Liver Pancreas Thymus Thyroid Parathyroid glands Epithelial lining of the gastrointestinal and respiratory tracts Epithelial lining of the tympanic cavity Epithelial lining of the urinary bladder and most of the urethra Pharyngotympanic or auditory tube Tonsils Tympanic antrum Blood Bone Cartilage Heart Blood vessels Striated and smooth muscles Lymphatic vessels Spleen Membranes of the body cavities (pericardium, pleura and peritoneum) Kidneys Genital ducts Ovaries Testis Connective tissue Cortex of the adrenal glands Mesoderm 1. Early development of human embryo - Tissues derived from three germ layers
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
A 1. Origin of human embryonic stem cells - Generation of hESCs by Immunosurgery Complement 1. Anti-human serum antibody Blastocyst 3. Physical plucking 2. Laser cut
Background image of page 6
Pluripotent human embryonic stem cells (hESC) Ectoderm • Skin • Brain Digestive tube • Thyroid cell • Lung Embryoid body (EB) Endoderm 1. Origin of human embryonic stem cells
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 12/11/2009 for the course BCH bch4122 taught by Professor Wang during the Spring '09 term at University of Ottawa.

Page1 / 33

LW_BCH4122_Lecture7_ - Lecture 7 Human Embryonic Stem Cells(hESC Lisheng WANG Department of Biochemistry Microbiology and Immunology Faculty of

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online