0303_5_Lewy - ORIGINAL ARTICLE Folia Neuropathol. Vol. 41,...

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www.fn.viamedica.pl 149 Folia Neuropathol. Vol. 41, No. 3, pp. 149–153 Copyright © 2003 Via Medica ISSN 1641–4640 ORIGINAL ARTICLE The morphological analysis of vasculature and angiogenic potential in meningiomas: immunoexpression of CD31 and VEGF antibodies Iwona Lewy-Trenda, Aleksandra Omulecka, Janusz Janczukowicz, Wielisław Papierz Laboratory of Neuropathology, Chair and Department of Pathomorphology, Medical University of Łódź, Łódź, Poland Meningiomas, despite being the mostly well-differentiated tumours, are quite often characterised by inva- sive growth and recurrences. As this fact may be caused by proangiogenic factors, we have investigated immunoexpression of CD31 and vascular endothelial growth factor (VEGF) in these tumours. The materials included 10 atypical meningiomas (G2), and the following subtypes of benign meningiomas (G1): 21 tran- sitional, 17 meningothelial and 13 fibrous. Paraffin sections were immunostained with CD31 (DAKO) and VEGF (Santa Cruz) antibodies. The highest number of blood vessels was revealed in atypical meningiomas and much lower (statistically significant difference) in benign ones. Diffuse or focal expression of VEGF of various intensity was shown both in atypical and benign types of tumours with no statistically significant differences between both groups of tumours and between subtypes of benign meningiomas. key w y w y w y w y wor or or ords: ds: ds: ds: ds: angiogenesis, vascular stroma, meningioma, morphometric investigation, CD31, VEGF Address for correspondence: Iwona Lewy-Trenda, MD, PhD Laboratory of Neuropathology Chair and Department of Pathomorphology, Medical University ul. Czechosłowacka 8/10, 92–412 Łódź, Poland tel: (+48 42) 679 01 91, fax: (+48 42) 679 01 91 INTRODUCTION A substantial role of angiogenesis in the progression of tumours has been postulated [3–6, 10]. Invasiveness is linked to proliferation of vasculature, whereas the in- hibition of angiogenesis may suppress growth of malig- nant neoplasms. Morphological studies of blood vessels in tumours of the central nervous system have been concentrated mostly on gliomas and metastatic tumours [12, 20, 21] and rarely on meningiomas [19, 25]. Here we present data concerning the vascular net- work and angiogenic potential of cells in the most com- mon histological subtypes of benign (WHO grade 1) and atypical (WHO grade 2) meningiomas. These tumours constitute the second major groups of primary CNS tumours [16, 18]. Although a majority of them are graded in the WHO classification [13] as benign (grade 1), their tendency to recur is observed. This tendency is more obvious in atypical meningioma [16]. MATERIAL AND METHODS The investigation was performed on sections of tu- mours from 61 patients (27 males and 34 females, aged 19 to 78, the average age was 53,3). Tumours were histologically diagnosed in accordance with the criteria of the WHO classification [13]. The study materials con- sisted of 10 atypical meningiomas (group 1) and be-
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This note was uploaded on 12/11/2009 for the course UNT 123456 taught by Professor 123456 during the Spring '09 term at University of North Texas Health Science Center.

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0303_5_Lewy - ORIGINAL ARTICLE Folia Neuropathol. Vol. 41,...

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