lecture18_09 - Announcements 1. Final exam, Tuesday,...

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Unformatted text preview: Announcements 1. Final exam, Tuesday, December 15 th 1:30-4:30pm, Room, TBA 4. Q and A, Wed, December 9th, 1-2pm 150 ASL 5. Ofce hours, December 8-11th, 14th, 3-4pm B521 CLSL 3. Last lecture, Monday, December 7th 2. Covers lectures 10-19 MCB 252, Part 2 Cell cycle III, lecture 18 CDK regulation degradation of Cyclin wee1, cdc25 Regulation of mitotic events Disassembly of nuclear envelope Initiation of anaphase Initiation of S phase G1 Cyclins in S. cerevisiae Conservation of key cell cycle regulators Biochemical puriFcation of MP from Xenopus (using 1 L of frog eggs yielded a few g MP) MP has H1 kinase activity Consists of two proteins (34 kDa and 46 kDa) What are they? Antibodies raised against conserved sequence in cdc2/CDC28 recognize 34 kDa band Antibodies against cyclin B recognize 46 kDa band So, MP is a heterodimer consisting of a Cyclin and a Cyclin-dependent kinase (CDK1) Pollard and Earnshaw, ig. 46-7 Structural models of human CDK2 Lodish, et al., Fig. 20-15 no Cyclin, T loop blocks access of substrates to active site: no activity + Cyclin, unphosphorylated: substrates can bind, minimal activity + Cyclin, phosphorylated: substrates can bind, high activity phosphorylation (activity) regulated by wee1, cdc25 Cyclin activity in Xenopus egg extracts RNAse-treated extract + cyclin B mRNA Pollard and Earnshaw, Fig. 43-10 + cyclin B mRNA encoding non-degradable form How is cyclin degraded?...
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This note was uploaded on 12/12/2009 for the course MCB 253 taught by Professor Wang during the Spring '09 term at University of Illinois at Urbana–Champaign.

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lecture18_09 - Announcements 1. Final exam, Tuesday,...

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