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Unformatted text preview: and the pH of the solution. 5.) Distilled water in equilibrium with air contains dissolved carbon dioxide at a concentration of 1.0 x 10-5 M. Calculate the pH of such a solution. 6.) Calculate the formal concentrations of acetic acid and sodium acetate necessary to prepare a buffer solution of pH 5.5 that is 0.2 M in total acetate. Extra Challenge Problem 7.) An enzymatic reaction takes place in a 10-mL solution that has a total citrate concentration of 120 mM and an initial pH of 7.00. During the reaction, 0.2 millimoles of acid (H + ) are produced. Calculate the final pH of the solution. What would the final pH of the solution be in the absence of the citrate buffer assuming that the other components of the solution have no significant buffering capacity and that the solution is initially at pH 7? Citrate p K a s = 3.13, 4.76, and 6.40....
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This note was uploaded on 12/13/2009 for the course BMB 461 taught by Professor Stoltzfus during the Fall '07 term at Michigan State University.
- Fall '07