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Unformatted text preview: 1 Outline of lecture 11 Fertility plasmid, bacterial conjugation, High frequency recombination Determining gene order from interrupted mating E. coli genetic map 2 RECOMBINATION AND MAPPING IN BACTERIA BACTERIAL CONJUGATION Plasmids are small, extrachromosomal, circular molecules in bacterial cells. They have a replication origin and often carry genes conferring resistance to antibiotics or provide a bacterial cell with other unusual properties. Episomes are large plasmid-like factors that can exist in two states: extrachromosomal integrated into the bacterial chromosome Fertility episome F F properties: (1) F carries close to 100 genes. It has a replication origin and replicates in synchrony with the bacterial chromosome. F replicates unidirectionally (rolling circle) during conjugation. (2) F + cells have pili for contact with other cells. (3) F + cells can transfer replicated F factor into F- cells....
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- Fall '08