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lecture_12 - 1 Lecture 12 Outline Mechanism of excision of...

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1 Lecture 12 Outline: Mechanism of excision of Hfr from the E. coli chromosome F’ episome. Origin of F’ episome by aberrant excision Merodiploidy Transformation Bacteriophage. Mapping phage genomes Bacteriophage λ lythic and lysogenic cycles P1 phage and generalized transduction Mapping bacterial chromosome with generalized transduction
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2 F' EPISOME F' originates by aberrant excision of Hfr episom by recombination between F and a flanking region of bacterial chromosome. A conjugation mediated by F' generates MERODIPLOIDY .
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3 Mechanism of F’ origin by aberrant excision
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BACTERIAL TRANSFORMATION Transformation results from recombination between exogenous DNA and the recipient chromosome MAPPING: DOUBLE TRANSFORMATION If a and b genes are far apart, probability of obtaining a double transformant is P 1 (transformation with a + ) and P 2 (transformation with b + ). The probability of
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This note was uploaded on 12/13/2009 for the course BIS 101 taught by Professor Simonchan during the Fall '08 term at UC Davis.

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lecture_12 - 1 Lecture 12 Outline Mechanism of excision of...

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