biochem test 4

biochem test 4 - 1/4? Final examination BlOiBCMB 3100...

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Unformatted text preview: 1/4? Final examination BlOiBCMB 3100 Friday, August 4. 2000 H4! What is the general function of membranes? in the case of mitochondria and chloroplasts, what more specific function do they have? “T; hmfit’auflj 4/ Ila-{J‘JJ/‘p/i‘wivéi/Q/‘flgfim " _’l j" ‘ . u / ' yv;.i"...-wj/-’lj. M1210 (frag/Z'KJ (aflf Mfl/GQK .- 1 be ("A/£64”, & £4. d’ZL/ A; __ ; .c/v 2.l3) Binding of ions to charged residues in proteins in water solutions is promoted by a change in what thermodynamic parameter? Explain. ., ; 1,:wa 3";L ‘.,__." ‘1‘ d'l/adf-«fl.f f ’f - . fl (Liv/(L? 3'}? .‘./ /{, ’21:; ’3’? it "‘7 “(j 'i/VLJi/Q-‘C-flvfi—Z'M- f: Hit-2:" (“I ../ a: . “- «vi/“w {-6 I.‘ g In I 7 I v (j gum. _ [/‘j of _ moo dog/Lu, ‘ Cifzx—C/ ’ ‘ . l" (7 m 3.(4l Glutathione is gammg-L-g|utamyl-L-cysteinylglycine. Draw the structure at pH 7. O (9’ 0 ‘1'- . j z) "" . H H w. r ___C #3” “d war rGH; ~c r-fi/fi‘fi: “C “'A/ 0/25 00 24 Li kl ’7 1 hi /"/ 2'- ,06} “h 1. 5.“) j ’1' . 33/3,.{zfl rfifi’ fl ‘_ 4J4} integral membrane proteins have what are called "transmembrane helices". What structural feature of o helices contributes to interaction with membrane lipids? ) _ I _ r ‘ '77”? 555/9 aim: :l’is SfFJC L; 0 L? {'{mtw Twit?- r.’ flat? 3 Giand/ idri’flf". 5i ((3. (1E.;€/i/O " ["0 if; ; 'tZEE' i‘. “(idea fit}; iii-1'9. 1/01.??- muff A f. id. (flyflée r5 I ,1: 1.1,: {91/ W: r. 85“ 6414) Competitive inhi ition can be overcome by increasing concentrations of g :4 fig ital:- {one word). The key assumption in.the - Michaelis-Menten model is formation of an C’m‘z, Im- e - SMEQZLPn’fF’: 325*; m ,g fiat (three words). In thgffiymotrypsin mechanism, the oxygen 0 Ber-109 re ate, with the carbonyl carbon of the substrate peptide bond to form a 153th 1 e. We (one word) intermediate. The negatively charged carbonyl oxygen is stabilized in the " x art} w (to a " {two words). T e substrate peptide bond breaks, orming an amine and a Q; Ma /z€?v: (one word) intermediate. This intermediate reacts with a may P-r‘ emf.» A” {one or two words) to produce a second . , .' _ .. , -. [one word) intermediate which breaks down to release the carboxylate product, leaving active chymotrypsin. 7.(5) Write the structure of thiamine perphosphatebTPP}. 0 [Fl Np: CHL‘CHZ-‘Ohl’br-"O;IF J—r *— flt, --"' . 4.» 0. é - I‘d/“f .flCH'z—N 8.!2} Circle the atom that is reactive when carrying out its function. 9.13) Given the reaction H-r‘i- oxaloacetate + NADH a malate + NAD+ WW 0/ Z Cai'culate AE‘“ +—2H"‘+2; “=2 raw/~76 11/ '/ a - ' Map/r7er 4-32— A0410 +24% «r Zr- H? mm, Emmi 4/4"" .,.._=,_ mm WZH” 25- f +05; "’ . ~r’ , - , _‘ - - - '+ ,, P +499!) W ,, 6. j 5am I oxa/om'VLafi/L? + Hwy flm‘q/ff 1013) Calculate AG”. Is the reaction spontaneous as written or not? 416%“ " - m 92:13” : HZ.- 23. ' 0J5 $367 Ara/Ami" _..- jfiS. Tabla 11-4 Standard reduction potentials for some important biological half-reactions Reduction half-reaCtion .'-:m (V) AcetleoA + C0, «- HC'B + 2:9 ——> Pymvate + CoA -043 Fen-admin (spinach). Fc®+ :9 -—-) Fe® —0.43 2H9 + 2:9 -—» H2 -0.42 u-Ketoglutant: + C03 + 2119 + 2:6 ——) hocim —0.38 Lipoyldenydmgenammm + 2HGB + 2:6 -—> Lipoyldehydmzenm {FADHQ -0.34 NADPQ + 2H6 + 2:9 —-> NADPH + H9 -0.32 NAD@ + 2149 + 2:9 ——p NADH + H0 —0.32 Lipoicacid + 21469 + 2:9 ——» Dihydrolipoic acid -0.29 Glumhione (oxidized) + 2363 + 2:6 -—> ZGIumhione (reduced) -0.23 m + 256) + 2:9 ——+ man, _ -0.22 M+2H®+2¢e -—+ W, -0.22 Amaldehyd: + 2 H9 + 2:9 ——» Emmi —0.20 mum+2HC9+2ze -—-> Lamps —0.:a 0111mm: + 2H0 + 2:9 —+ Milne ~01? Cytochrome b, (mammal). Fe®+ :9 -—+ Fe® 0.02 Emanu+2H®+2ee --—r Succinlne 0.03 UbiquinoneiQ) + 21-163 + 2:3 -—> QH, 0.04 Cytochzorrnblmitochondnd).l=e®+ .9 -—-> p.33 0.00 Cytochrome c.. Fe® + :9 -—) Fe® 0.22 -Cy10¢ltromec.Fe®+ :9 ——D Fe® 0.23 -Cytoctlmrne a. Fe®+ :6 -—-> Fe@ 0.29 Cytochmef. Fe®+ ea ——> Fe® 0.36 00,9 + 2 He + 2:63 ——:- N0,9+ H10 0.42 Mystem P700 0.43 Fe®+ :9 ——9 F993 0.17 Ix2014. 2H®+ 2:9 ——» up 0.32 x 11.!6} Write the Haworth structures of lactose, sucrose and fl-D-ribofuranose. 3‘01»? O “I f O W” / 0 0H h C" £31? l / a l Cl M HO lat.“ 12.[10) What bonds hold the two molecules together: a) glycosidic b} ester c) anhydride d) hydrogen e) peptide. An amino acid to a tRNA 5 An amino acid to AMP in amino acyl adenylate . Glycerol to a fatty acid in glycerides é; . Adenosine to phosphate in a nucleotide [Z . Glucose to fructose in sucrose xcz/ . A pyrimidine to ribose in a nucleic acid é» . Alanine to arginine in a peptide Q . Serine to the phosphate of a phospholipid f . One peptide chain to another . The complimentary bases in double stranded DNA Q . 1348) Briefly describe the series of reactions which convert D-glucose to acetyl TP A L) r I w. . 4 61%: case Glfzrmscéf’ .57; /}z_{__(c.?f05¢ g > 7:. _. . \ .* _ N140 ~;. 0 F)? a C #0360: 5;. A/g/D {2; 940 ,0" J ADP 47p emwflewswwe p . w.( 4 fit/C; 0,194” w’ W> / cal-’1;- 06) flew/x " fir?) III/I, FC 0/7’ :12;- #6 as: \/'> €675” <0; I? My Met may féfi I w I C M;- C r F ngtfif/ Mlgfl/a/ n ; (’5’ #0 3 ' ’ . CH 2. {F 10611 .X Péf} FE F (1/5 1416) Briefly describe the Citric Acid Cycle. What is the function of this pathway? How are intermediates in the Citric Acid Cycle maintained in the fed animal and during starvation? F o x 022$??? CH 55cm 096611 (Jo-cc {a {sf CH 33 C 3" ‘i” A 3.. “x '50 260”; Grind I; {ride / “:3; WWW FADiiZZmAe*ir/(\NADH+ H- atmfidrcoz‘i — / N/im- ,.....§‘ . he a? { L50 C 1 L2: 9:? E, 9 y H 0g 51. L N49 léwww f? +6’Xafwflc 67$;ng K—FHOH 9” K’gf [Maya E21115 quc mtg, /C,x. I, flab/mws xx flit-’13 H0 6w? d l: FADH‘ (9H2) r ‘1- NADH+ H “’4 60,2 f'ff')1-(_.//flf%;z:( 14¢ .- i: H ‘) :“C‘O 6/3 . " _ “NI/2 . r- a. {462,1 r£((j 04 736?! [hiking Q-Mciymlf 6 “(z/($7,,- E‘A 11;, f g 3/? Oi‘i'_:‘r._.?m€zf 922.1% (“:43 lei-2.54;, {riff/(LC { Jsfi. 15.!5) Briefly describe the pentose phosphate pathway and indicate two functions of this pathway. "T‘iu Goat. (a +— N A W” a N16} £2}'>'H -/ H' 7‘- 6 7—.AMfl40 flaw/fiat” é 'W'£”»‘--'/w‘6f {1W w“??? ar—N/iwigie ,,..o"/‘9"/0/7/ 1" 2" (599.2 "‘ ARM)“. f; ,5 547" Warm/Kde 7‘, fl/zfimfiosf 5W A”; 5&5..£ :3 x" . r I ADM/'95:“ .{;I/i-‘7/-_ C, 5;“- 5/3 ' ' " w/ 64/559741”ch _€$’/D¥—}dp/;/,fk/JSK/o /z.fl/2.cfi( 4/9429/19 c {3%}. u, c, x35...( 5/2,; 5mm“; {fa/[Ar 9,35 4') , . . A, I , . . 9 9’ L I if [’4 if (C if“)? J'gdu-‘affi id 15 //Vr’77fl/0 J } ""\ _ \ If .. ../ ( p7- v P 1646) Briefly describe oxidative phosphorylation. How are ADP and Pi converted to ATP and H20 during the transfer of electrons from NADH + H+ to 1/202 to form NAD+ and [V é; in. —. ’3 4 a_ __W__t~, 0 A a a_/ J a f? z fl’:?:’2(;g. V (2’ ‘4‘ .5 f _wmfi f w_ _ Miro If! 7:» Hf—J} “T? "_é‘;”m a, £532 +,..:’ I 5 (7/4??? SW1?» I ‘5, r : ‘1‘.’\..I.*:"LO‘J 1'} H" Hf 5! if I HI'QQ “a” 1/ -- J i. - I #2" {6L y1-¢.4-’.--; ( LL?- -‘ n g. z c r“ i? H % 17.!4) Briefly indicate the reactions involved in the synthesis of triacylglycerol and phosphoiipid. (:21 I," ' :3. .31; _ ' _, 3712 (“W .. -. // '“' ‘3 ‘7 A OH P a 5'" P C :5” (-1” IT." 5' fit] 0 I (j C «J ,‘l I; " m an. f‘ C 00 // 7L ,Q [a w} by“: (r I I W ... N6 0177‘ H " / ‘ i ,\, (V2069 {2/ C 5 Ma 1,, C E\ {’lfl'W/ata ) ( of {a 9 aim/ntfi ) fl, 0 CH 2 0 C 13 I; F R C o C ’ H 71 C “520 WW awfsz Ada/j) 18.14} Write a sequence of reactions which will oxidize CH3{CH2)n COOH to CH3(CH2)m,2, gSCoA and acetyl SCoA. q o (1% (ac/Q 35in 4‘- 247794-- 62» (“VS/7’ -—;; flr-Kf’f f7} f (1/4? @fi’zfl-IT‘QI/ ./' h H (9 3 (Cf/2J- ,.<". #2 CH3 a 5&2}? + F/M w> [film-f.” 4.. (15%;; agar-Haggai r z I”? j C?) 30 77' Z 3i " 2; 9* *C-H C u: f? 4 flit-- “é Wm 0H was; r" ,4? W} b ;. ff _ ,.- ._.2 \.._:. a _ CH CH, ‘53". , I“ - )7" ‘9 ’7‘ g {‘2 )7]; fit/2w“ ,9 r? +A/HD—9 Nflwz’ 1‘ rag/Chi Owl 5253/] (mm a" _. 0 7" L" 5 4. "(lapse H7L _‘ ' " 71_ 2 0 4fl3flr>[email protected]+cw;mmifl M) f" FW-v-‘ZI- 19.!4) Write a sequence of reactions which will convert acetyl SCoA and malonyl SCoA to the 4 carbon acid derivative butyryl SACP and 002. CH 9 c 9 36 J C; A + Ad (we; dig} 05 my -+- Ca; A g H (r? _ _ _, c7 #006, mg 33%qu p _._1 -2 w K‘ E; —~ " . fé <.'.;f.:_r'ifc:7 555.; ;# ‘jK/Jfiffi"; fl, 5’0 H A {f It? 6‘; ,. . m... . {QCS/gCJ/g Cf ‘5 W“;- C £77-i/3f-f"* .r‘v::--i:-.»‘li f" “In; / if“ 1/ a f g ’0‘ i. Icky? I C76,}; “fl [fir f. L 20.(8) Give the initial four stages or reactions involved in light energy capture by all photosynthetic organisms. ' l ({C' I" (‘1 :nf'Ei‘; (my C[‘. {O fl (#4 (Mo-€39” ml 9:»: -.. a) r - K 21 .(3) What are the chemical products of photosynthesis {light reactions only) in green plants? - 22.(4) What is the main distinguishing characteristic of the photosynthetic apparatus in photosynthetic bacteria such as the purple sulfur bacteria and in green plants? /C w Zwtfix‘a / y’qu/ [vi/1):”, 23.l4} What two chemical reactions, according to your text, produce the pH gradient? 2 “7—”? 02f “7" + + far-nor (L “1* ZS“ 1‘ 2 Half we”) a/Vo/OM Ilium 1-; 24.l2) What is the chemical name of the acceptor for CO2 in all photosynthetic organisms? fr'éu/Oée- /; 5 25.(4) In general terms. how do some groups of plants overcome the inherent oxygenase activity of rubisco? flu: efledzm COA (.Dmcfnnjl’d= Your 6113612”)ch “Ki/2r: Halal-"Sci; [5- ,‘gcrmgw/i n» (Affiff? ft) 02378:; éj J5;st yaks'rw aka/vacflla‘é ffmfi p57?) (MAO/502: ) flea/L {rams ‘Or'ZL-x'mc ’om /Oc? (Bf—“74?- ”fo _ _ a? (75 W}va méfsgoj {£631 L7,? 0x0? 26412) Briefly diagram the replication fork and indicate which DNA strand is synthesized continuously and discontinuousiy. Be sure to indicate the 3' and 5' end of daughter strands and the proteins or enzymes involved in forming and stabilizing this structure. Cw (fizwuoaw (figfidzrim? Sofia mfg) ,.. f3!" (Mm.._-z..__. .. _.._fl_d-,.z_..-___..___m_WM ____ 5.5-} ._ 5’ F _:c' l.) - f / i O f fiz‘iiijwcazi‘ 1"” «7 ’ ' r , 3 r i! \J/ 06'; (“it if ({b V! Q gffl‘tit'fi “LIL JLC—a-“CJQ— (- I If" -, z — u §/__fl F g ’- flm‘a “w ) 0/qu . . . ’ 161.45. I 9x16, (if). (‘ {Ar-1- ; L ( 6:, fié “’3 .5 I I In . - A a? (M w 57’)? fl : at.” J —»£6(/J «sf/2m J; / j I {rife-«n 3‘ ' j. 0M4? I_ fizz-<21? at... 0 $0430”; git-(.- Vflm‘f' fan-m. agar Mm: z-JZ‘I 7/7? I: H “ff/3‘62. 1 id?» d _ O. 27.(15) Describe the reactions and functions of Primase. DNA polymerase Ill and DNA polymerase I (Exoribonuclease) in the synthesis of DNA. _. A739 .. 9M 7/ (5‘77; _;#_{._.._...> “ON 7.".(‘2‘Ajzzlijnjz f): [fl/Z/WL/ x'Zéf-‘j m T. (IL-y, j I I . .’ *' flmrrrz flaw If I, eta-r} ‘3' l‘ i. g . .. 1r": _- _i ." V4 p _. r. Jr???” ‘DA/fl?” +5677? —> fi/Wf' (WAN—0m). 4— pa. J 5 c1“ 77/" ' 5i 77/? pm yméymwmd. 33 a 544;” 5'00 74’ 2m 566.er 6/1914! ('0 z."- .P’xm’g Jo fiflflwflzmtfl-g DAM ARA/131} “AM/ if 0" 'Ew _ ,.~—/~'JP 7( (6)7, ——7‘~ pA/fl( BMW)” 4 6M!) ",4 Mg’thr‘afi Z— [email protected]fiha’ Q'CJWfi-éy arcs-(.2 p/d-"zrrtf- '5- ’? j 28.115) Briefly list the postranscriptional processing of mRNA and tRNA before they can function in protein synthesis. . 2% m4 '6 raw-"21;? (Ix/1,2027?” f: g “((6 M55? @500 A. /Zjié’w~:r’7fam~bv /---g;_...{; 6-5,- " ' " AA: fates/4:." Z6 . I fijyiga dig/aback V a“ C._ fl «2- \. I} f I J, ,..-a I I ( r?“ I {r A 'r. Z d“, .2 _ x.” It + r t a x :5 fl / L; 29.(6) What are the three characteristics of the genetic code for protein synthesis (one word each)? Md”) 2 (Jr: 1’54 / 5".”7‘ 30.{4) Describe what any two of these mean. f« 5(J.‘m--~{~ m flrjar‘flfmf 6””"V'Or ru'XC'W-flbyfij Z i is". t? C? “4:052 336:"; 2/16: S 5.61,)?!“ caJ‘) fl 5 3‘ Swazi; flim-rf n/ 524) 53%5’5 w «r2 / /‘ J 31MB) Match: 8. ubiquitin f; initiation complex b. EF-Tu L fMet tRNA binding 0. Mg2+ _L_ A site d. 238 RNA i translocase e. lF—2 . a. protein degradation f. EF-G a), l peptidyl transferase g. diphtheria toxin _h_ establish correct reading frame (procaryotesl h. Shine-Dalgarno sequence 51 eukaryotic protein synthesis i. ribozyme 32.(2) Approximately how many equivalents of ATP are consumed to form one residue in a protein? 33.l6) The structure of urea is given: 0 ll HzN-ONH2 From what compounds are the two nitrogens and the carbon derived in the urea che? A 1H4- dqq dng .4 ,-{r‘ “I. Hot/3 34.l4l Nitrogen fixation uses ATP. How is the ATP employed in reduction of N2? A;.0/JUA.3/j (“5 60; 7/1/96/ 7‘0 fi'cf/a/(f'o Tr’(/”‘X whiff??? “0:515; {PW/7.714515: “50kg; "SHEFWP finish: a Efraij ‘rm/HGW'I“? ...
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This note was uploaded on 12/14/2009 for the course BCMB 3100 taught by Professor Mendicino during the Spring '07 term at UGA.

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biochem test 4 - 1/4? Final examination BlOiBCMB 3100...

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