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Unformatted text preview: CHAPTER 20 DNA TECHNOLOGY OUTLINE I. DNA Cloning A. DNA technology makes it possible to clone commercial applications: an overview B. Restriction enzymes are used to make recombinant C. Genes can be cloned in recombinant DNA vectors: D. Cloned genes are stored in DNA libraries E. The polymerase chain reaction (PCR) clones DNA genes for basic research and DNA a closer look entirely in vitro Analysis of Cloned DNA A. Restriction fragment analysis detects DNA differences that affect restriction sites B. Entire genomes can be mapped at the DNA level Practical Applications of DNA Technology A. DNA technology is reshaping medicine and the pharmaceutical industry B. DNA technology offers forensic, environmental, and agricultural applications c. DNA technology raises important safety and ethical questions OBJECTTVES After reading this chapter and attending lecture, the student should be able to: 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2. Describe the natural function of restriction enzymes' 3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA I I . I I I . fragments. 4. Explain how the creation of sticky recombinant DNA molecule. 5. Outline the procedures for producing plasmid and phage vectors' 6 . E x p l a i n h o w v e c t o r s a r e u s e d i n r e c o m b i n a n t D N A t e c h n o l o g y . T " L i s t a n d d e s c r i b e t h e t w o m a j o r S o u r c e s o f g e n e s f o r c l o n i n g . g. Describe the function of reverse transcriptase in retroviruses and explain how they are useful in recombinant DNA technology' 9. Describe how "genes of interest" can be identified with the use of a probe' 10. Explain the importance of DNA synthesis and sequencing to modern studies of eukaryotic genomes. I 1. Describe how bacteria can be induced to produce eukaryotic gene products' 12. List some advantages for using yeast in the production of gene products. 13. List and describe four complementary approaches used to map the human genome' ends by restriction enzymes is useful in producing a 296 unit III Genetics 14. Explain how RFLP analysis and PCR can be applied to the Human Genome Project. 15. Describe how recombinant DNA technology can have medical applications such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products. 16. Describe how gene manipulation has practical applications for agriculture. 17. Describe how plant genes can be manipulated using the Ti plasmid carried by Agrobacterium as a vector. 18. Explain how foreign DNA may be transferred into monocotyledonous plants. 19. Describe how recombinant DNA studies and the biotechnology industry are regulated with regards to safety and policy matters....
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- Fall '09
- Trigraph, recombinantD NA, na t echnology, ecombinantD NA