02-Spectrophotometers

02-Spectrophotometers - BSCI330 Cell biology and physiology...

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BSCI330 – Cell biology and physiology Fall 2009 LAB MANUAL – Lab Exercise #2 Carpenter et al. 2009. BSCI330 Laboratory Manual. University of Maryland, College Park. 1 EXERCISE 2: SPECTROPHOTOMETERS Review of Laboratory Technique From your previous experience in other biology courses, you should have been introduced to several important laboratory techniques. Although they should therefore already be familiar, we wish to review them briefly, as optimal success in many of the laboratory exercises this semester will depend upon their proper implementation. 1. Use of the spectrophotometer Spectrophotometry is a useful technique that can be used to follow the course of biological events by monitoring the change in light absorbance or transmission over time. We will be using the spectrophotometers in several of the labs this semester. The spectrophotometer is a comparatively simple device, basically consisting of a light source and a filter. Various chemical compounds exhibit absorbance of light energy at certain wavelengths due to the characteristics of their quantum structure. By monitoring the absorbance (more accurately the optical density or OD), the concentration of the compound can be accurately determined. Recall that
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This note was uploaded on 12/15/2009 for the course BSCI 330 taught by Professor Payne during the Spring '08 term at Maryland.

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02-Spectrophotometers - BSCI330 Cell biology and physiology...

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