BISC320SI2009midterm2

BISC320SI2009midterm2 - www.usc.edu/si Casey Williamson...

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www.usc.edu/si BISC 320-Molecular Biology Casey Williamson Scott Greenberg cwwillia@usc.edu sagreenb@usc.edu Molecular Biology SI Exam Review #2 10/21/09 Things to review on your own : - DNA pols, Proofreading exonuclease, Primer removal on Lagging Strand, ddNTPs, 3 factor crosses, Loss of heterozygosity/ Rb, Mating Type Determination (basics) 1. Draw a Eukaryotic replication bubble and explain how replication facilitates the cellular repair machinery to identify the newly synthesized strand incase of improper base insertion. 2. A) What are the requirements of a PCR reaction and B) draw the steps to show how you obtain the exact product length desired? 3. Why are PCR primers usually ~20 bases in length? 4. What is the function of telomerase? a) Give its specific biochemical activity b) Describe its substrate
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www.usc.edu/si BISC 320-Molecular Biology Casey Williamson Scott Greenberg cwwillia@usc.edu sagreenb@usc.edu c) How is it targeted to that substrate? 5. The following DNA molecule contains a mismatch (underlined). Name the DNA repair
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This note was uploaded on 12/15/2009 for the course BISC 13019 at USC.

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BISC320SI2009midterm2 - www.usc.edu/si Casey Williamson...

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