PCR short tandem repeat

PCR short tandem repeat - 99 PCR amplify

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Short Tandem Repeat (STR) Polymorphism Type 1 Primer 1 ----------> 62 ------------------ATTG.ATTG.(ATTG) .ATTG.ATTG------------------- 62 ------------------TAAC.TAAC.(TAAC) .TAAC.TAAC------------------- <---------- Primer 2 99 PCR amplify -------------------------------------------------- -------------------------------------------------- PCR product = (2 x 23) + (66 x 4) = 46 + 264 = 310 basepairs Type 2 Primer 1 ----------> 66 ------------------ATTG.ATTG.(ATTG) .ATTG.ATTG------------------- 66 ------------------TAAC.TAAC.(TAAC) .TAAC.TAAC------------------- <---------- Primer 2
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Unformatted text preview: 99 PCR amplify ----------------------------------------------------------------------------------------------------PCR product = (2 x 23) + (70 x 4) = 46 + 280 = 326 basepairs. The DNA template is DNA from an individual's cells and could be millions of bps long. Assume both primers are 23 bases long; they must be unique sequences and not repeated elsewhere in the organism's DNA. Primers 1 and 2 will bind to different strands. Heating the DNA allows the primers to bind. The dots are included to make it easier to see the repeat sequences....
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This note was uploaded on 12/16/2009 for the course BIOLOGY 2112 taught by Professor Rappaport during the Fall '08 term at Temple.

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