BICD110development

BICD110development - Carbon supply during germination...

Info iconThis preview shows pages 1–15. Sign up to view the full content.

View Full Document Right Arrow Icon
Starvation
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Infectious agent of: Anthrax (Bacillus anthracis) Tetanus (Clostridium tetani) Gangrene (Clostridium perfringens) Diarrheal diseases (Bacillus and Clostridium sp.) BT insecticides (Bacillus thuringiensis) Resistant to: Desiccation, heat, radiation Germicides, antibiotics Endospore Dormant bacterial cell Ubiquitous in nature - soils, spices, etc Botulism (Clostridium botulinum.)
Background image of page 2
forespore Spore mother cell Dies The endospore: a cell within a cell The endospore allows spore assembly to occur in the cytoplasm
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Coat (protein) Cortex (peptidoglycan) Adam Driks, Loyola University Core (dehydrated cytoplasm)
Background image of page 4
Exosporium (lipids, proteins, ?) Coat (protein) Cortex (peptidoglycan) Some spores have an extra external layer: Crystaline Insecticidal toxin
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Key features of the core: 1. Partially dehydrated (10-30% of normal H2O) •Increases resistance to Heat, Germicides •Inactivates enzymes Lots of: 2. Calcium Dipicolinic acid (DPA) •Found ONLY in endospores 3. SASP (small acid soluble proteins)
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 14
Background image of page 15
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Carbon supply during germination Protect DNA from UV, dessication, heat Spore DNA (DAPI stained) The SASP change the shape of the DNA spore Germinating spore Growing cell Manasherob et al., AEM 64:1750- Spore germination Steps in germination: 1. Sensing of germinant (food) Triggers rehydration, which allows enzymes to be active. RNA & Protein synthesis Degradation of SASP Release of Ca+DPA 2. Swelling and outgrowth. Steps in sporulation: 1. Initiation Move division site to pole Turn off other developmental fates : Competence Motility Biofilm formation Degradative enzymes 2. Polar septation Small forespore Large mother cell Growth Sporulation forespore mother cell Engulfment: phagocytosis in bacteria Proposal: Cell wall hydrolases can act as motor proteins The cell wall can serve as an external cytoskeleton forespore Spore mother cell Dies How do you make the endospore?...
View Full Document

Page1 / 15

BICD110development - Carbon supply during germination...

This preview shows document pages 1 - 15. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online