BIO325 quiz 2007 - GENETICS {BIO 1325} Summer 2007 i J ‘...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: GENETICS {BIO 1325} Summer 2007 i J ‘ x I Quiz 6/6/07 Total points: 10 The chromosomes in a eukaryotic cell can be observed clearly during metaphase ofmitosis and the entire collection of chromosomes in a cell is referred to as the karyotype. Since most eukaryotes contain a large number of chromosomes, (for example, humans have 22 pairs of autosomes and a pair of sex chromosomes in somatic cells), how would you distinguish the different chromosomes in a chromosome set? Mention any twofeatures that would allow you to identify each chromosome in a chromosome set. (2 points) ' . . ._. , H Um “ti; tutti? ‘: 0W (0 01/ out the amrvétLei-é: CIMWUwu/W’ UM L triawwxvfic \ 0\‘_.ql (I bzy’mjt WHAOW’ * WjfltzthJ/‘lw4( A 57": 5'”? ‘5" g . a, . ..-,, “1.; ,’ ,MV mm; a U m 0 idwj‘w’ai wsz f N Wu? “a: “Vi—‘4. /\ S‘SHW pin in lll‘lqh/i"? vi N» Jam 55..“ _ (a) Identify the most likely mode of 1nher1tance (Aut mal recesswe; Autosonpl/do/minant; X-linked recessive; X-linked dominant; Wed; Mitochondrlal) 1n the followmg pedigree. (2 pomtsLfi/ x . .-’ l ‘; ' ~i! / t / x / " ,. l w x“ / l ' g 1: ‘ an/ 1 g 2 ‘ x YA x“ " .1 n L“ --U Ll L; O “1‘ 3 1 ° 3 4 5 6 7 i a 1 s ‘ i m It , xn ox A m M iii . w‘ X 1‘: X 1"“; W I l u: 1 2 3 ' a 5 a 7 £3 9 10 (b) What is the probability that individual IV-9 will exhibit the trait indicated in this pedigree? (2 points) Lo) (21) (b) Identify the most likely mode of inheritance (Autosomal recessive; AutoWant; X-linked recessive; X-linlggdxkfififrfint; VifiRe/d; Mitochondrial) in the following pedigree. (2 points) n l raw: ,e i 1 ? 4 s 6 l i t" l « m M w (j: i M U I 9 z 2 3 4 5 . 6 WW What is the probability that individual IV-6 will exhibit the trait indicated in this pedigree? (2 points) 7f M. Li} 3g; GENETICS {BIO 1325) Summer 2007 , ,i. , ’T VH u é : 1] Quiz 6/15/07 Total points: 10 1. At time zero. an Hfr strain was mixed with an F' strain, and at various times after mixing, samples were removed and agitated to separate conjugating cells. The cross may be written as Hrra+ b+ c+ d+ e+f+ g+ h+ StrS x F'a' b- c- d' e'f' g- h- Str” —> F ’ (no order is implied in the above listing) The samples were then plated onto selective media to measure the frequency of h+ Strr recombinants that had received certain genes from the Hfr cell. The following graph shows the percentage of recombinants against time in minutes. 100 + h+ + 75 __ e 0/08er recombinants 50 __ b+ 9+ 25 __ 3+ 0 5 10 15 20 25 Time In minutes Based on the given information, answer if the following statements are true or false. (4 points) (i) All F ' cells that received a+ from Hfr in the chromosome transfer process must ave received b’. 7%! (ii) The or of gene transfer from Hfr to F' was a0 (first), then g: th I) then e+, then h+. F a (E ? ost e7 Strr recombinants are likely to be Hfr cells. ( a HQ a (iii) iv None of the b? Strr recombinants plated at 15 minutes are also a . ’(VM Two E. coli strainsrwere Obtained with the following genotypes: . + .s s NIHMV Hfr: ala leu/aZi Str l t / t I J R yo _ + - _R Fga/la, leu azr Str . v u o + After an unmterrupted conjugation, you want to select exconj ugants that are ala + I ‘leu . Which of the following media would you use for this selection? (2 points) (. Complete medium containing streptomycin a l 4 l ( V; / 6' Minimal medium containing streptomycin @Minimal medium containing leucine and streptomycin (d) Minimal medium containing sodium azide and leucine (6) Minimal medium containing alanine, leucine, and streptomycin 3. Hfr strains are frequently used in time-of-entry mapping in E. coli. Using the information presented in the table below, construct a single time-of-entry map for the genes indicated and show the location of the F factor and the direction of transfer for each Hfr strain. (4 points) GENETICS (BIO 1325) 06/20/07 SUMMER 2007 Total points: 10 (6 points) III Quiz 1. Fill in the following blanks: (b) During DNA replication, the dau er strand that is called the 0M“. V1 {H (A , and the discontinuously is known as the nthesized continuously is and that is synthesized a w d (c) If DNA polymerase adds an incorrect nucleoti e o the 3’ terminus, a separate catalytic domain containing 2113’ to 5’ (H) Ki 7 f activity removes the mismatched base. (d) lnitiationlof DNA 5 t W1 (7 it uses r bonucleotide tri thesis on the lagging strand require short made by an enzyme called QV l W“ {e , which ’osphates as substrates. (e) The unwinding 0 he DNA helix at the replication fork is catalyzed by a m ll (6!! 9 {Which uses the energy from ATP hydrolysis to move unidirection y along (g) Replication bubbl s are formed at special DNA sequences called ' W of“! (MW: OwC \ I A"? “W? W15} :01; \J _ 2. E. coli cells are put into a medium containing tritiated (3 H) thymidine for one generation only. The cells are then transferred back to normal medium with nonradioactive thymidine. After one generation in the normal medium, what proportion of the cells will be labeled? (2 points) enzyme telomerase adds repeat sequences to the ends of linear eukaryotic omosomes. This enzyme behaves as a: (2 points) (a) DNA-dependent DNA polymerase DNA-dependent RNA polymerase RNA-dependent DNA polymerase ,(é) RNA-dependent RNA polymerase <§<a < §<<< 31mm ACT 661 Me. ATE) (ac—rs' «WWW S'ch Tem- Céxfi' 1m 1m (gm 3/ W 6“ V F i O i V‘ (A \ W" \ \ L‘L \ N 7 a‘: v‘ ‘ / f \ C K WH P , J) J > \IL/>7, 3. IV Quiz Tit/74w (law “new U6 g GENETICS (B10 1325) SUMMER 2007 06/28/07 Total points: 10 For each of the following E. coli merodiploid strains containing the lac operon alleles listed, indicate whether B-galactosidase and permease activities in these strains is inducible, constitutive, or uninducible. (5 points) fi-galactosidase W (a) 1+ P+o*z*Y*/l‘ P*o°z*Y' (W m WW mallet W7 (b) . I+P*o*Z'Y+/1*P*o°ztv* Co‘fmmh‘fl Cottmmhw I'P‘O+Z+Y'/I+P+OCZ'Y+ immaculth U cal/let httttWQ (d) lSP*o*z*Y*/1‘P+o*z*Y' Wit/Wattth W“ “WU l? u (e) 15 P+o*z*Y+/l' P+O°Z+Y' W5 «Witth LWI Malta MU List any two differences in regulation of transcription between eukaryotes and bafsterifints) , . , t, d‘ (2 p» m w Harm ’ mth trim/MC lfv‘fifwétgw Wmfl/fioé wave/(Um iv} Ct/tlZWI/Lj ’W/ Wmhfiw A Cmijomf MC {.61 CM .‘/g mth WW ‘ WNW/m ’ (W l. + 0N inc/Mg, ‘0; Le] mug/21 l/ await \m t/utllwétjctt’f " W0 W mottfcgti‘ W“ W “Had How are most eukaryotic gene regulatory proteins able to affect transcription when their binding sites are far from the promoter? Select ONE answer. (2.5 points) By binding to their binding site and sliding to the site of RNA polymerase promoter By unwinding the DNA between their binding site and the promoter / assembly @ By looping out the intervening DNA between their binding site and the (d) By attracting RNA polymerase and modifying it before it can bind to the promoter WW7 M t7 (xi/7m GENETICS (BIO 1325) Summer 2007 xfl/H S 3 V Quiz 7/3/07 Total points: 10 b For each of the following sentences, fill in the blanks with the best word or phrase selected from the list below. Not all words or phrases will be used; each word or phrase should be used only once. (4 points) Restriction enzymes that cut DNA straight across the double helix produce fragments of DNA /with M MT {Nd . A fragment of DNA is inserted into a / g 9 {Jaw/l in order to be cloned in bacteria. A C DN library contains a collection of DNA clones derived fromij As. A C’JQl/IW/ll C library contains a collection of DN ones derived from chromosomal DNA. blunt ends DNA polymerase RNA cDNA staggered ends DNA ligase probe vector genomic List any two of the three features that a cloning vector for use in bacteria must contain. _ « (2 points) ~ OW (ll 1“ . . . . //V I Sm (fl m m. 0H fill mg Ltd «Stiff — Wm Z1 LW /’ SWWWU WWW/0?] You are given the plasmid pUC18 for cloning DNA fragments. An EcoRI fragment was cloned in the unique EcoRI site in this plasmid vector and the DNA mixture was used for transformation of E. coli cells that were deficient in B- galactosidase activity. Following transformation, the cells were plated on medium with the chromogenic substrate X-Gal and the antibiotic agricfln. (4 points) (\i) What will be the color (blue or white) ofthe E. coli colonies if cells in ’ these colonies have the plasmid carrying the insert DNA? \0 Wet (ii) Will the colonies be ampicillin resistant or sensitive ifthe cells carry the plasmid with the insert DNA? ( yr t \ l W M ...
View Full Document

Page1 / 8

BIO325 quiz 2007 - GENETICS {BIO 1325} Summer 2007 i J ‘...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online