eukGeneRegulation

eukGeneRegulation - Regulation of Gene Expression in...

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Regulation of Gene Expression in Eukaryotes Chapter 18
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Outline How we use genetics to study gene regulation Using mutations to identify cis -acting elements and trans- acting proteins How genes are regulated at the initiation of transcription Three polymerases recognize three classes of promoters Trans-acting proteins control class II promoters Chromatin structure and DNA methylation regulate gene expression How genes are regulated after transcription
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Regulatory elements that map near a gene are cis -acting DNA sequences cis -acting elements Promoter – very close to gene’s initiation site Enhancer (or silencer) • Can lie far way from gene Can be reversed • Increases (or represses) basal transcription Fig. 18.1 a
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Reporter constructs are a tool for studying gene regulation Sequence of DNA containing gene’s postulated regulatory region, but not coding region Coding region replaced with easily identifiable product such as β -galactosidase or green fluorescent protein Reporter constructs can help identify promoters and enhancers by using in vitro mutagenesis to systematically alter the presumptive regulatory region
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Regulatory elements that map far from a gene are trans -acting DNA sequences Genes that encode
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eukGeneRegulation - Regulation of Gene Expression in...

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