geneticEngr1

geneticEngr1 - Genetic Engineering Techniques(Part 1...

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Unformatted text preview: Genetic Engineering Techniques (Part 1) Chapter 9 Deconstructing the Genome: DNA at High Resolution Recombinant DNA technology is used to carry out the following basic operations Cut enormously long strings of DNA into much smaller fragments with restriction enzymes and separate them by gel electrophoresis Isolate, amplify, and purify fragments through molecular cloning Use purified DNA fragments as hybridization probes to identify similar sequences in libraries of clones or mixtures of DNA or RNA molecules Rapidly isolate and amplify previously defined genomic or mRNA sequences through the polymerase chain reaction (PCR) Determine the precise sequence of bases within isolated DNA fragments Fragmenting complex genome into pieces • Restriction enzymes fragment the genome at specific sites Fig. 9.1 Restriction enzymes cut DNA molecules at specific locations (restriction sites) and produce either blunt or sticky ends Fig. 9.2 Fig. 9.3 The number of base pairs a restriction enzyme recognizes determines the average distance between sites and thus the size of fragments produced Partial digests are used so enzymes cut only a subset of the total number of recognition sites in a genome Fig. 9.4 Gel electrophoresis separates DNA fragments according to size...
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This note was uploaded on 12/17/2009 for the course BIO 2354 taught by Professor Brockett during the Spring '08 term at Georgia Tech.

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geneticEngr1 - Genetic Engineering Techniques(Part 1...

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