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Discussion questions 5 - KEY

Discussion questions 5 - KEY - Discussion questions 4 1...

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Discussion questions 4 1. Transcription of gene A takes place from left to right. Transcription of gene B takes place from right to left. Which strand of DNA (top or bottom) as shown in the following figure serves as the template for transcription of each gene? 5’ 3’ Gene A = bottom; Gene B = top 2. The following DNA sequence contains the start site of transcription of a bacterial gene. The sequences shown in bold represent the –10 (TATATT) and –35 (TTGACA) regions of the promoter. 5’CGTGGACCTACGTAC AATATA AGCTAGCCCTAGTCGAT TGTCAA CGGTACCGATCTAAT-3’ 3’GCACCTGGATGCATG TTATAT TCGATCGGGATCAGCTA ACAGTT GCCATGGCTAGATTA-5’ (a) Will the start site (+1) for RNA synthesis be to the left, right, or between the two sequences shown in bold? LEFT (b) Which of the two strands (upper or lower) will be the template strand? UPPER 3. The following segment of DNA occurs within the protein-coding region of a gene: 5'–GGAACTCTAGGGGCTG–3' 3'–CCTTGAGATCCCCGAC–5' Which one of the following is true: A) The upper strand must be the transcribed strand.
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