1Tues_Introduction - Introduction to the Microbiology Lab...

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Introduction to the Microbiology Lab Welcome to Microbiology at Miramar College! We look forward to a rewarding and enlightening experience with you all. Because our labs and protocols involve not only living organisms, but also some that are potential pathogens, you must adhere to strict safety guidelines and rules, in order to ensure safety for yourself, your colleagues, and your family and friends at home. The safety rules and guidelines are set forth below: 1. Lab coats are encouraged but not required and must be kept in personal boxes. 2. Closed-toed shoes are required for protection from spills and accidents. 3. Long hair must be tied back during lab. 4. Wash hands before you begin work and before you leave. In addition, it is a good idea to wear gloves for most lab exercises. 5. Wash down lab bench with disinfectant before and after work. 6. No extraneous books, folders, purses, or book bags on lab bench top. 7. Report all spills and accidents. Be prepared to identify the microbe as the instructor requests. (See Hazardous Waste Protocols) 8. No cultures in boxes at any time, and no removal of any culture, plate, or test tube from the lab. 9. Test tubes will be transported in a test tube rack at all times. These rules will ensure a good working environment for everyone. Additional protocols and equipment lists are outlined below. HAZARDOUS WASTE DISPOSAL All materials that have come in contact with a microbial organism are considered hazardous waste and must be treated by the following guidelines. Hazardous waste disposal (The Morgue) - Test tubes: Always place test tubes in the appropriate test tube rack in the morgue. There are 2 types of tubes used (13mm and 16mm) and separate size racks are provided for each. Completely remove all media labels before placing in the rack. - Glass: Glass items such as slides and coverslips need to be placed in the yellow sharps container in the morgue. - Additional sharps: Other sharp objects, such as pipets and pipet tips, toothpicks, and cotton swabs, need to be discarded in the white tub. - Non-sharps material: All other contaminated material, including plates, gloves, and paper towels are discarded in the large, plastic Bio-Bag in the morgue.
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- Stains: All stains must be collected for disposal and NOT poured down the sink. Staining trays can be emptied into the large white carboy labeled “Stains Collection”
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This note was uploaded on 12/23/2009 for the course BIO 205 taught by Professor Murphy during the Fall '09 term at Miramar College.

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1Tues_Introduction - Introduction to the Microbiology Lab...

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