2Thurs_Motility - Lab Exercise 10: Microbial motility...

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Lab Exercise 10: Microbial motility OBJECTIVES 1. Make a hanging-drop preparation for viewing live microorganisms. 2. Understand principle behind motility media and be able to determine motility based on inoculated results. INTRODUCTION The major organelles of motility in bacteria are flagella . These structures allow cells to move toward or away from a stimulus in the environment, through a process known as taxis . The stimulus might be chemical in nature, such as a nutrient, acid, or noxious chemical, and the process is then referred to as chemotaxis . If the stimulus is light, the process is referred to as phototaxis . There are different arrangements of flagella on the cell surface, and these arrangements are useful for characterization of the organism. (Please see Chapter 4 of your text for more descriptions of flagella arrangements) Motility can be determined by several methods. Microscopically, one can observe motility using a type of wet mount known as a hanging drop slide , whereby a drop of viable cells is placed on a microscope cover slip, which is then inverted on a microscope slide. This technique reduces the effect of drying, although hanging drop slides should be observed shortly after their construction. For the beginner, true motility under the microscope must be differentiated from
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This note was uploaded on 12/23/2009 for the course BIO 205 taught by Professor Murphy during the Fall '09 term at Miramar College.

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2Thurs_Motility - Lab Exercise 10: Microbial motility...

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