4Thurs_Lethal effects of temperature

4Thurs_Lethal effects of temperature - Lab Exercise: Lethal...

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Lab Exercise: Lethal effects of temperature OBJECTIVES 1. To predict and determine the thermal death point (TDP) and thermal death time (TDT) for various organisms based on physiological and ecological characteristics. INTRODUCTION Many factors affect how a cell will respond to temperature. Organisms that are found in extremely warm environments, such as Thermus aquaticus, will have adapted to produce enzymes capable of withstanding heat, such as Taq polymerase. Some species are able to produce heat stabile spores which will help them survive periods of extreme heat even when the vegetative cell would not thrive. Additional factors such as pH, moisture, composition of medium, and age of cells will also influence the results. Standard methods for determining susceptibility to temperature are the thermal death point (TDP) and thermal death time (TDT). TDP is the temperature at which the cell is killed in 10 minutes. TDT is the time required to kill a suspension of cells or spores at a given temperature. LAB EXERCISES
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4Thurs_Lethal effects of temperature - Lab Exercise: Lethal...

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