4Tues_Oxygen effects on growth

4Tues_Oxygen - Lab Exercise 12 Effects of oxygen on growth OBJECTIVES 1 To differentiate between oxygen requirements using anaerobic culture media

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Unformatted text preview: Lab Exercise 12: Effects of oxygen on growth OBJECTIVES 1. To differentiate between oxygen requirements using anaerobic culture media. INTRODUCTION Microbes can be classified into 5 groups based on their requirement for air that contains 20% oxygen: 1. Obligate aerobes: Require oxygen for growth via respiratory metabolism in which oxygen is the terminal electron acceptor. 2. Obligate anaerobes: Can not survive in the presence of oxygen due to lack of enzymes superoxide and catalase or peroxidase, which detoxify damaging oxygen by- products such as peroxides and superoxides. Use fermentation or anaerobic respiration. 3. Facultative anaerobes: Can grow in oxygen or without. Use oxidative respiration and fermentation/ anaerobic respiration. 4. Microaerophiles: Require small amounts of oxygen (5-10%) for respiration but can not survive in atmospheric oxygen due to limited ability to carry out respiration or presence of oxygen-sensitive enzymes. 5. Aerotolerant anaerobes: Can survive in oxygen due to presence of superoxide dismutase but do not use oxygen as a terminal electron acceptor. Use fermentation or anaerobic respiration. The oxygen requirements of a given organism can be easily determined in the microbiology laboratory by the use of both solid and liquid media. microbiology laboratory by the use of both solid and liquid media....
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This note was uploaded on 12/23/2009 for the course BIO 205 taught by Professor Murphy during the Fall '09 term at Miramar College.

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4Tues_Oxygen - Lab Exercise 12 Effects of oxygen on growth OBJECTIVES 1 To differentiate between oxygen requirements using anaerobic culture media

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