4Tues_ph effects on growth

4Tues_ph effects on growth - Lab Exercise Effects of pH on...

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Lab Exercise: Effects of pH on growth OBJECTIVES 1. To determine optimum pH for growth for organisms with different characteristics INTRODUCTION pH is an important environmental condition to consider, as an excess of either hydrogen ions ( H + ), as exists in an acidic environment or an excess of hydroxyl ions ( OH - ), or other H + acceptor, as exists in an alkaline or basic environment can be equally damaging to cells not adapted to survival in those conditions. Either extreme will denature membrane proteins and quickly cause the death of the cell. While the vast majority of eukaryotes are categorized as neutrophiles , enjoying an environmental pH close to 7.0, many bacteria require acidic ( acidophiles ) or alkaline ( alkalinophiles ) conditions to grow or even survive. It is important to note that although many bacteria are obligate acidophiles or obligate alkalinophiles, most all bacteria maintain an intracellular environment near a neutral pH of 7. pH requirements for growth can be easily tested by
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