8Tues_Diversity- bacteriophage

8Tues_Diversity- bacteriophage - Lab Exercise: Diversity of

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Lab Exercise: Diversity of Microbes- Bacteriophages OBJECTIVES 1. To understand the lytic cycle of bacteriophages 2. To be able to make serial dilutions. 3. To be able to determine concentrations of viral samples from PFU counts on plates. INTRODUCTION Viruses are very small acellular entities. Bacteriophages are viruses that infect bacteria. They appear to “eat” up bacteria. The term “phage” by itself means "eat" (as in phagocytosis), and generally refers to a bacteriophage virus. Most bacteria have phages that are able to parasitize them. In fact, the ability to be infected with a known phage type is used to identify some strains of bacteria. Usually a phage is very specific to a bacteria species or stain of a species. We will be working with a T-even phage that only infects E. coli strain B. We will also use a phage called Phi ( φ ), which only infects E. coli strain C. As the virus infects bacterial cells, the infection destroys the bacteria. See your textbook for more on lytic cycle of bacteriophages. The bacteria have been
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This note was uploaded on 12/23/2009 for the course BIO 205 taught by Professor Murphy during the Fall '09 term at Miramar College.

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8Tues_Diversity- bacteriophage - Lab Exercise: Diversity of

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