lab_01_introduction - Miramar College Biology 205...

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Lab Exercise 1: Microbiological Safety & Laboratory Introduction Page 1 of 4 Miramar College Biology 205 Microbiology Lab Exercise 1: Microbiological Safety & Laboratory Introduction Welcome to Microbiology at Miramar College, we look forward to a rewarding and enlightening experience with you all. Because our labs and protocols involve living organisms that may be potential pathogens, you must adhere to strict safety guidelines and rules in order to ensure safety for yourself, your colleagues and your family and friends at home. The safety rules and guidelines listed below will ensure a good working environment for everyone. Additionally, you will find a detailed explanation of the proper placement and care of laboratory equipment; waste; and organisms. Safety Rules & Guidelines 1. Lab coats are encouraged but not required and must be kept in personal boxes. 2. Closed-toed shoes are required for protection from spills and accidents. 3. Long hair must be tied back during lab. 4. Wash your hands before you begin work and before you leave. 5. Wash down your lab bench with disinfectant before class begins and after you have cleaned up your supplies. 6. Remove all extraneous books, folders, purses or book bags from the lab bench top. 7. Report all spills and accidents. Be prepared to identify the microbe as the instructor requests. (See Hazardous Waste Disposal protocols) 8. Do not store any cultures in your personal or community box. Never remove any culture, plate or test tube from the lab whether or not it has been inoculated with an organism. 9. Transport test tubes in a test tube rack at all times. Figure 1: Diagram of the laboratory. Morgue areas include a Bio-bag (1) for disposal of non-sharps waste; small and large test tube racks (2); a sharps container for disposal of microscope slides and sharp plastics; and a large plastic carboy (4) for stain disposal. Student benches are labeled table 1, table 2 and table 3 (left to right) and contain 8 seats. The first aid area contains a shower and a first aid box. A fire extinguisher is located in the front left hand corner of the laboratory. Storage for student “personal boxes” is in the upper cabinets, as are all of the reagents. Students are not allowed in the autoclave and media storage rooms. Hazardous Waste Handling All materials that have come in contact with a microbial organism are considered hazardous waste and must be disposed of according to the following guidelines. Hazardous waste disposal (The Morgue)
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This note was uploaded on 12/23/2009 for the course BIO 205 taught by Professor Murphy during the Fall '09 term at Miramar College.

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lab_01_introduction - Miramar College Biology 205...

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