lab quiz - Planes of sectioning: Plant organs are commonly...

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Planes of sectioning: Plant organs are commonly sectioned in two different planes: transverse and longitudinal. Longitudinal sections may be in two different planes, one along the radius of the organ and the other at a right angle, or tangent, to the radial longitudinal plane. TS (or XS)- transverse or cross section LS- longitudinal section RLS- radial longitudinal section TLS- tangential longitudinal section CRT- a prepared slide with cross, radial, and tangential sections Cytochemical staining protocols: Assume all stains are toxic. Keep stain off your skin. All of these will stain clothing. Toluidine blue: Method A: 1. Immerse sections in toluidine blue solution for 15-30 seconds. 2. Rinse sections in water. 3. Transfer sections to drop water on microscope slide and add a coverslip. Method B: 1. Add a small drop of toluidine blue to drop of water on a microscope. 2. Add sections to the drop on the slide and then add a coverslip. Results: A good metachromatic stain. Lignified walls and tannin-containing structures tend to stain blue-green or green. Cell walls of nonlignified parenchyma, collenchyma, sieve-tube members, and companion cells tend to stain pinkish-purple
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to red. Over staining turns everything blue. This is not a definitive stain. For example,
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This note was uploaded on 12/25/2009 for the course PLB 9876655 taught by Professor Canington during the Spring '09 term at UC Davis.

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lab quiz - Planes of sectioning: Plant organs are commonly...

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