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BIO 325 - Fall 2009 - TTh 1230 to 200 - Quiz 3 key-1

BIO 325 - Fall 2009 - TTh 1230 to 200 - Quiz 3 key-1 - from...

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GENETICS (BIO 325) Fall 2009 T, Th 12:30 – 2:00 PM section Key - Quiz 3 __________________________________________________________________________________ 1. (a) Plasmids Bacteriophage vectors Cosmids Bacterial artificial chromosome (b) A cloning vector for use in bacteria must contain the following: 1. a bacterial replication origin (to allow the plasmid to be replicated) 2. at least one unique restriction site (to allow easy insertion of foreign DNA) 3. an antibiotic-resistance gene or some other selectable marker gene (to allow selection for bacteria that have taken up the recombinant plasmids) 2. (a) A genomic library is prepared from total genomic DNA; a cDNA library is prepared
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Unformatted text preview: from mRNA. (b) A genomic library contains much more information than a cDNA library. (c) A genomic library prepared using DNA isolated from different cells or tissues would be the same whereas a cDNA library will be different for different cells or tissues. 3. Forward primer 5'-TCGATT-3' and reverse primer 5'-CCGTAA-3' 4. (a) (i) Rsa I produces blunt ends (ii) Xho I produces sticky ends with 5' overhang (iii) Pvu I produces sticky ends with 3' overhang (b) Yes. Both enzymes create DNA fragments with compatible GATC sticky ends. 5. (a) True (b) False (c) False (d) True (e) False...
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