This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: GENETICS (BIO 325) Fall 2009 Discussion sheet 9 key _____________________________________________________________________________ _ 1. Gene regulatory proteins are generally used in combinations, thereby increasing the possible regulatory repertoire of gene expression with a limited number of proteins. 2. (i) GAL4 (ii) GAL3 mutants in the presence of galactose - not expressed GAL80 mutants in the absence of galactose - expressed 3. Added hormone Estrogen-inducible genes Progesterone-inducible genes Estrogen not induced not induced Progesterone induced not induced 4. Bam H1 and Bgl II; 5'-GATC-3' 5. Some cohesive ends can form perfect Watson-Crick pairs, and therefore can be ligated; others cannot. Ligations (1) and (2), each involving two ends created by a single restriction enzyme ( Bam H1 and Xba I, respectively), regenerate a restriction site for the corresponding enzyme. Ligation (4) is successful because the cohesive ends are compatible, but ligation creates a "hybrid" hexanucleotide sequence that is not a...
View Full Document