Discussion 8 Fall 2009

Discussion 8 Fall 2009 - GENETICS(BIO 325 Discussion sheet...

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GENETICS (BIO 325) FALL 2009 Discussion sheet 8 _____________________________________________________________________________ _ 1. What structural characteristic of an operon ensures that the genes contained in the operon will be coordinately controlled? 2. Consider an E. coli cell with the following mutations. What effect would each mutation have on the function of the lac operon (assuming no glucose is present)? (a) A mutant lac operator that cannot bind repressor (b) A mutant lac repressor that cannot bind the lac operator (c) A mutant lac repressor that cannot bind to allolactose (d) A mutant lac promoter that cannot bind CAP plus cAMP 3. Positive and negative control are different ways of controlling the activity of a gene. What is the difference, and can one gene be subject to both modes of control? 4. In the table below, state whether or not the genetic regions of the lac operon listed are transcribed in wild-type cells of E. coli: Transcribed in Transcribed in presence of lactose absence of lactose (a) Z gene ____ ____ (b) I gene ____ ____ (c) lac promoter ____ ____ (d) Y gene ____ ____ 5. Would the following genotypes for the E. coli lac operon be non-inducible (non-ind), inducible
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This note was uploaded on 12/30/2009 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas.

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Discussion 8 Fall 2009 - GENETICS(BIO 325 Discussion sheet...

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