Problem set 7 answers2007

Problem set 7 answers2007 - Answers: Problem Set 7 2007...

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Answers: Problem Set 7 2007 Problem 1. A. Wild type Tg. #1 Tg.#2 Ig Mc       IgMc        Ig Mc Tg BS A B. Wild type Tg.#1 Tg.#2 F1 IgMc IgMc IgMc IgMc IgDc        IgDc            IgDc       IgDc Ce ll # Cell# C. Bone marrow Lymph Node Thymus Peripheral blood B2 2 0       B2 2 0 CD 4 CD8 CD8 D. Bone marrow Lymph node Thymus
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Answers: Problem Set 7 2007 Peripheral blood B2 2 0       B2 2 0 Tg BS A CD 4 CD8 CD8 E. Since the Surface bound antigen (BSA) is only present in the liver (i.e. the periphery) the F1 mouse will still contain B cells displaying the transgenic antibody when we look in the BM. Once the maturing b cells enter the periphery and come into contact with the cell surface bound BSA, the Abs on the surface of our B cells will be crosslinked which kills the cells in the absence of CD40L and leads to deletion in the periphery. Since this mouse is not transgenic for a specific TCR, the flow profile for CD4 and CD8 is not visibly affected in the thymus and peripheral blood (even though the T cells in this mouse will be tolerant to BSA). F. Transgenic spleen cells: Anti-DNP antibodies Anti- BSA antibodies Tg. #1 - + Tg. #2 - - G. Tg. #1: No anti-DNP antibodies because Tg.#1 is already transgenic for a particular heavy and light chain that are specific for BSA. No other ab will be made by B cells in the Tg. #1 mouse. BSA ab will be made because the #1 Tg. has B cells and T cells specific for BSA which will be primed in vivo by the DNP immunization, and then restimulated by the subsequent invitro exposure. Tg. #2: Although the Tg. #2 mouse does have DNP specific B cells, they won't be properly primed because of a lack of BSA specific TCEll help in this mouse, which will be tolerant (both B and T cells) to BSA because of the high levels in the serum. So without BSA specific Tcell help
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This note was uploaded on 12/31/2009 for the course MIMG 185 taught by Professor Zack during the Fall '06 term at UCLA.

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Problem set 7 answers2007 - Answers: Problem Set 7 2007...

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