BIBC 100 Chymo Mech

BIBC 100 Chymo Mech - 2 3. The first product of the...

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Chymotrypsin Mechanism Mimi Nguyen BIBC 100 Fall 2009 1 1. Substrate binds enzyme a. Side chain goes into specificity pocket b. Reactive Ser195 in active site that attacks carbonyl C of substrate c. Forming of unstable intermediate 2. Intermediate-tetrahedral negatively charged transition state (acetyl-intermediate) a. Reformation of double bond b. Peptide cleaved
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Chymotrypsin Mechanism Mimi Nguyen BIBC 100 Fall 2009
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Unformatted text preview: 2 3. The first product of the reaction leaves a. Water enters and attacks carbonyl C 4. Second unstable intermediate formed a. Nucleophilic attack of water forms next negatively-charged tetrahedral transition state 5. Double bond reformed and enzyme regenerated a. Covalent bond between substrate C and Ser O is broken, releasing the second product...
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This note was uploaded on 01/02/2010 for the course BIBC BIBC 100 taught by Professor Buehler during the Fall '09 term at UCSD.

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BIBC 100 Chymo Mech - 2 3. The first product of the...

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