UCSDDNA1 - Discovery of DNA structure o Rosalind Franklin...

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Discovery of DNA structure o Rosalind Franklin used X-ray crystallography to determine that DNA was a helical model
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Watson and Crick constructed models based upon this data and determined that DNA consists of two intertwined DNA molecules with the structure described above
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DNA structure and Replication DNA is made up of nucleotide monomers Each nucleotide monomer is made up of : sugar (deoxyribose) nucleotide base (Adenine (A),Guanine (G),Cytosine(C), or Thymine(T)) phosphate group
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Nucleotide: A closer look Nucleotides of DNA and RNA contain a phosphate group
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Nucleotide: A closer look Nucleotides of DNA contain 5c sugar: Deoxyribose RNA contain a contain 5c sugar: ribose
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Nucleotide: A closer look Nitrogen-containing bases classified either as Purines: A and G found in both DNA and RNA
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Nucleotide: A closer look Nitrogen-containing bases classified either as Purines: C found in both DNA and RNA T in DNA U in RNA
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Nucleotide: A closer look… Put these three components together….
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Linking nucleotides together to make polynucleotide strands… Phosphodiester bond linking 5’phosphate with 3’ sugar of next nucleotide
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DNA secondary structure DNA consists of two polynucleotid e strands arranged as a double-helix sugar- phosphate backbone that make up the rails
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Complementary bases pairing up using hydrogen bonds make up the rails A denine: T hymine Two H bonds G uanine:Cytosine 3 H bonds DNA secondary structure
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Chargaff’s rules: A=T;G=C…so (A+G)/ (T+C) =1
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DNA secondary structure The two strands are arranged antiparallel One strand begins with a 3’ deoxyribose sugar group and ends with a 5’phosphate. The other strand begins with the 5’phosphate and ends with a 3’deoxyribose sugar group
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RNA Single polynucleotide strand Ribose sugar Uses U in place of T So A on a DNA or RNA molecule will pair with U on RNA
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RNA Complementary bases within single- stranded RNA molecules often pair forming complex secondary structures These include hairpin consisting of a stem of complemenary bases and a loop of non complementary bases
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DNA secondary structures B-form Alpha helix;clockwise spiral Predominates under most cellular conditions including abundance of water
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DNA secondary structures B-form 10 bp turn Produces minor and major grooves that proteins like transcription factors or restriction endonucleases can bind
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DNA secondary structures A-form Smaller,wider Conditions of little water Little physiological evidence
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UCSDDNA1 - Discovery of DNA structure o Rosalind Franklin...

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