2009 Midterm2 key

2009 Midterm2 key - K M 1. Glycolysis regulation (23 points...

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Unformatted text preview: K M 1. Glycolysis regulation (23 points total) 1a Please draw a curve that shows PFK-l activity (as Y-axis) against ATP concentration (as X—axis) in the absence of Fructose-2, 6—bisphosphate (2 points). Draw another curve in the same plot that shows PFK~1 activity (as Y—axis) against ATP concentration in the presences of 20 14M Fructose—2, 6—bisphosphate (2 points). WM FLIG I317 0 1b. In the following graph, PFK—l activity (y—axis) is plotted against Fructose—6—phosphate concentrations (x— axis). The only difference between the two curves is ATP concentration. Which curve represents the reaction with higher [ATP]? (3 points). Mark the Km values of PFKl for Fructose—6—phosphate in the graph (2 points). Can you estimate the Km value of PFKI for ATP from this graph (1 point)? NU/ we what atmte Km. PFK—I activity IFructosgdphosphatel _ af F Z 5 5P& “1’ butt tar/{NW f H (if; 1d. Briefly describe how low blood glucose level slows down g colysis (4 points) [31¢le —-+ swam? ——, cmrta mm A wk? .4 Pmyyfa ——o oggivfi a F' L" m l iv '5’!” Lye” r hm i W" /" "' (Quiz 0 ‘ 5 1e. Write the structure of Fructose—2,6—Bisphosphate (3 points) 14 ".0 ‘ H (F; If. What are the negative regulators for PFKI (4 taunts)? 0" “f ATP , Gil-n 1g. Fructose—1,6—Bisphosphate is a positive regulator for glycolysis. What is the target enzyme for this compound (2 points)? Po‘OM/Al-l/ lawms" 2 2. Converting 6-carbon sugar to S-corbon sugar (10 points). 23. What is the enzyme for the following reaction (2 points)? 0‘1 : row» cm I ‘ 0;; . a , m H.M.__(A:._."OH aldolaSO F LQ,_..,,_,A(II:W_H g 1 er r: on + 0H w » agH + ; 9 i H-WCW / -r OH r: «WOH , 2-: E Ell-30W)?“ CHZOPOE“ I ‘~ , f akin” ‘f 2993 $ 2b. Whatis favism (4 points)? A Coan'i’on ch Whit/‘1 a‘xrson (ad; Glmcc—é PllefllW/L Wamrc, Wl’. .M.F¢V‘ 5%: WM PmX/M m 179 Blvwl kiln/Marc awn/id! (“5/ Am» (11‘, Koo. 626(1) an m ADM: QM €274: Cal rho/u, W {Lt/5 Mats 0n all-m ths I tnZ’lc“W‘hzit—ist—lfexfzme of the athwa that co ert 6" b0 0 tHS 'b suoa (2 ointg)9 plus/1A4: Pun“ .. p y nv s ~car nsugar o —ca1 on g r p . is I 1 AAA firs Penlow Wasp ltqu Paflw‘j 2d What are the physiological functions of this pathway (2 points)? {'0 77mm” NM?” 55 F'M‘CS'Q 3. Pyruvate dehydrogenase complex (14 points) 3a. In addition to TPP, What are the other cofactors in p ruvateélehydrogenase complex (4 points). Li? A I L/po/o a“ / FAD/NAN 3b. Please define the “flexible arm” in pyruvate dehydrogenase complex (2 points). Please provide another example that uses a flexible arm (2 points). A 01mm “Wand of UFO/z, Mid f 0-, 9w OLWEML wavm “Waxy/m 3c. TPP is a key cofactor for pyruvate dehydrogenase complex. TPP is also used in other enzymes. Please name the other three enzymes that also use TPP as a cofactor and that were discussedin the lectures (3 points). WWW, [win/“WK Way/«w, xtolehthmk Wham. 3d. Electrons are transferred from pyruvate to A and then to B and finally to C in pyruvate dehydrogenase complex catalyzed reaction. What are the names for A, B, and C, respectively? (3 points) A; 0,17,)“, “11/1 or ‘I‘pom’olb [3: F140 0-5/va 4. The following questions are related to TCA cycle (17 points total). a). Who discovered the TCA cycle (1 point)? And where the discovery of TCA cycle was published (2 Points)? ml, 3 EA Wm OLD m b) What are the enzymes that catalyze the anapleroti reactions for TCA cycle (4 points) I) PvIFWlf/ levxvlm Z PEP Mom/lam 7) PEP “('00va My", 3‘1) Mam, Ea’ejmc c) What is the pathway that can convert acetyl CoA to succinate without releasing C02 (2 points) 114. filfioxr,’ Patna-my d) Why isocitrate lyase is a good drug taroet for treating some bacteria infections in humans (2 points) 1+ 8 not amt m M; t impofiw-i’ ‘Fw‘ Mm. 9 ma Swami-a...» e) Why citrate synthase can hydrolyze citryl-CoA, but not acety'l—COA (Use fewer than 2 sentences to answer the question) (3p0ints) D ONI’NJ/{V‘k Ml” 3‘” "M h‘ “‘7”- 7, 11-0 «11W“ “MM WW1 3r Maw/15,3 Muslim (he, f) What is the TCA cycle enzyme tha uses Fe—S cluster for non-redox reaction (2 points)? W" I "0’4 Await-L Le ’5 50"“ g) Who proposed the “three—point contact” hypothesis (1 point)? Al—CYMM Ogslcn 5. Oxidative phosphorylation (16 points) a) Draw a schematic picture of a mitochondrion and clearly label the membranes and compartments (3 points). Indicate where oxida e phosphorylation a1 a ‘ cycle take place (2 points) W MM WW mm M Q 9 ’ ’Wm‘“ ‘IM" c) Explain what is the “Q cycle” and how it is related to the establishment of a proton gradient across the inner membrane (4 points) TM) a S; to: o g ()9, +9.“, [4* R.“ {ume l Pf: l OW'AL.“ 2694+}, % nah (My, Muse, L! H“ +9 0719501 [pl—s. 05.3%”: Z (BM 611%, d) What could be the chemicals for A, B, and C in the following figure? (3 points) A: Zak/tom. B: Anfimjcm A c. 3 CO or CAI '- e) Which compound / intermediate in the figure has the lowest redox potent'al? (2 points) NA!) t) Compound A inhibits electron transport from NADH to Q. How to determine experimentally whether the inhibitor can block the oxidation of NADH (2 points) - at 4», 0 [a x AB, W N9 01”) r 7, “a.” n5 ’i‘ “M M3“ (Way; Aq-Z'm) P :5 ywim‘wm my 390nm aHWbH. A (X) NADH ———~> Q--~>Cytb —> Cytcl —~—>Cytc -->Cyt(a+a3)—-~> 02 B (X) NADH -——>Q -—> Cytb ——9 Cyt c1 --—-> Cytc ——> Cyt (a +_a3) -—> 02 C (X) NADH—fiQflCytbflCytcl—fiCytc ———~>Cyt(a+a3)——~>02 6. The following questions are based on the following four compounds (20 points total). - f - O O— OOC\C/O lcoo okc/OH \C/ l :«zo—c—H ( 1 CH2 \ I , ' H—C—‘OH HO—C~—H H C——H | [CH2 1 l 2— CH3 COO COO_ CH2OPO3 A B C D 6a When 1 molecule of A is converted to 1 molecule of B 6211) How many substrate level phosphorylation reactions are involved (2 points)? 1 6212) How many dehydrogenases are required (2 points)? 2 6213) How many NET NADH are produced? (2 points) ‘ 6214) How many NET FADHZ are produced? (2 points) l 6a5) How many NET water molecules are used? (2 points) | 6b When 1 molecule of C is converted to 1 molecule of D 6b1)How many substrate level phosphorylation reactions are involved? (2 points)? | 6b2) How many dehydrogenases are required (2 points)? \ 6b3) How many NET NADH are produced? (2 points) 69 £0 S R v I r 6b4) How many NET FADH2 are produced? (2 points) 0 Germ/r MS'WU @(fp 6b5) How many NET water molecules are used? (2 points) @‘l 6/ THE END ...
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2009 Midterm2 key - K M 1. Glycolysis regulation (23 points...

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