Biomolecules Lecture_11

Biomolecules Lecture_11 - Biomolecules and Metabolism...

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Lecture 11 - Enzymes Biomolecules and Metabolism
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Overview Enzymes - Classification - Regulation - Mechanism - Proteases - Kinetics/Assay
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Kinetics 1. Study of the rates of enzyme catalyzed reactions 2. Gives information about enzyme catalysis, enzyme mechanism, regulation of enzyme activity (i.e. inhibitors, activators), basis of enzyme assays 3. Kinetics are affected by [enzyme], [substrate], pH, temperature, coenzymes, activators, and inhibitors
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Basic Kinetics E + S ! ES " E + P Kinetics 1. When [S] >> [E], every enzyme binds a molecule of substrate (enzyme is saturated with substrate) 2. Under these conditions the rate depends only upon [E], and the reaction is pseudo-first order
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Basic Kinetics 1. Fixed, saturating [S] ( zero order ) 2. Pseudo-first order enzyme-catalyzed reaction 3. Reaction rate is independent of [S] Kinetics
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Basic Kinetics 1. The initial velocity (V 0 ) is the slope of the initial portion of the curve 2. Rate of the reaction doubles when twice as much enzyme is used Kinetics
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Biomolecules Lecture_11 - Biomolecules and Metabolism...

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