BE230_Lecture_26 - Cell Structure & Function BE230 Dr...

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Unformatted text preview: Cell Structure & Function BE230 Dr Christine Loscher Lecture 15 Flow Cytometry Flow cytometry is a method for quantitating components or structural features of cells primarily by optical means Although it makes measurements on one cell at a time, it can process thousands of cells in a few seconds Since different cell types can be distinquished by quantitating structural features, flow cytometry can be used to count cells of different types in a mixture. Flow Cytometry The cells may be alive or fixed at the time of measurement, but must be in monodisperse (single cell) suspension They are passed single-file through a laser beam by continuous flow of a fine stream of the suspension Each cell scatters some of the laser light, and also emits fluorescent light excited by the laser The cytometer typically measures several parameters simultaneously for each cell: - low angle forward scatter intensity, approximately proportional to cell diameter - orthogonal (90 degree) scatter intensity, approximately...
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This note was uploaded on 01/05/2010 for the course FSH BT taught by Professor Ianmarison during the Fall '09 term at Dublin City University.

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BE230_Lecture_26 - Cell Structure & Function BE230 Dr...

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