BE230_Lecture_3 - BE230 Week 2 Lecture 4 The Red blood cell...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
BE230 Week 2 Lecture 4 The Red blood cell membrane & Transport Dr Rosaleen Devery School of Biotechnology
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Week 1 You learned about components and structural organisation of cell membranes
Background image of page 2
Lecture 4 The Red Blood Cell An example of plasma membrane structure Chapter 4 in Cell & Molecular Biology Concepts & Experiments 3 rd edition Gerald Karp Page 147-150 Transport of ions & small molecules Chapter 7 in Molecular Cell biology 5 th Edition Lodish, Berk, Matsudaira, Kaiser, Krieger, Scott, Zipursky Darnell Page 245-252
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
At end of today’s lecture You will be able to describe a model of erythrocyte plasma membrane Describe structure and function of its integral proteins and its peripheral proteins Compare and contrast permeability of pure phospholipid bilayer to gases, ethanol, water, glucose, fructose, ions (K + , Mg ++ , Ca ++ , Cl - , HCO 3 - , HPO 4 - ) Outline how ATP-powered pumps, ion channels, and various transporters (eg uniporters, symporters, antiporters) function Outline how uniport transport differs from passive diffusion Describe how GLUT1 functions Describe an experiment to study how transport proteins function Describe 4 classes of ATP-powered transport proteins
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
The RBC example Inexpensive to obtain Readily available in huge numbers Single cells No nuclear or cytoplasmic membranes Intact plasma membranes by placing in hypotonic salt Cells take up H 2 O, swell hemolysis Hb content flows out leaving plasma membrane ‘ghost’ Solubilise and Fractionate proteins (SDS PAGE) Enzymes (eg glyceraldehyde 3 phosphate dehydrogenase), transport proteins (for ions, sugars, O 2 , CO 2 ), skeletal proteins
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Integral proteins of RBC Glycophorin A Large number of negative charges borne on sialic acid RBCs repel each other Prevents cells from clumping as they circulate thro’ tiny blood vessels Receptor used by protozoa that causes malaria- how it enters blood cell If lack glycophorin A & B, you are protected from acquiring malaria Differences in amino acid sequence determine whether you are MM, MN or NN blood type Band 3 Channel for passive exchange of anions HCO 3 - enters in exchange for Cl - (in tissues)
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Peripheral proteins of RBC Located on internal surface Determine biconcave shape Restrain movement of integral proteins
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 12
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/05/2010 for the course FSH BT taught by Professor Ianmarison during the Fall '09 term at Dublin City University.

Page1 / 63

BE230_Lecture_3 - BE230 Week 2 Lecture 4 The Red blood cell...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online