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Chapter 6b Worksheet - Biology 310 Worksheet Chapter 6...

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Biology 310 Worksheet Chapter 6 Worksheet 2 Key Concepts -What is the Michaelis-Mentin Kinetic? What reaction does it describe? -(Know the graph of the kinetic) -What is V MAX ? -What is K M ? (Hint: Think in terms of V MAX and substrate concentration.) -How can V 0 be calculated? -What is the Lineweaver-Burk plot? What does it show? -Know how to draw it!!!! -Know what the values of the intercepts mean. -How is V MAX calculated when an enzyme is saturated? -Does changing the concentration of enzyme change K M ? -What equation is used to measure catalytic efficiency? -When does catalytic efficiency equal the rate constant for the formation of the enzyme-substrate complex? -There are two possible schemes for reactions involving more then one substrate. Know what they are and know the differences between them. -There are 4 types of enzyme inhibition. -__________________________________ -__________________________________ -__________________________________ -__________________________________ -Know how each method of inhibition functions and know how they affect K M , V MAX , and how these changes would look on a Lineweaver-Burk plot.
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