8 - Sorting of newly-made proteins Fall 2009 SF

8 - Sorting of newly-made proteins Fall 2009 SF - Sorting...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Sorting newly-produced proteins o Pancreatic model o Assembly of secretory vesicles o Signal sequences o Integral membrane proteins o Core glycosylation o Sorting lysosomal enzymes o Transfer into mitochondria o Movements through the nuclear envelope Pancreatic acinar cell secretory vesicles rough ER Golgi complex intermediate vesicles
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Protein synthesis in eukaryotes ¾ Free ribosomes ¾ ER-bound ribosomes ¾ Pulse-Chase Studies 1) Slices of pig pancreas 2) Incubated with radioactive amino acid 3) Traced the path of newly-made proteins in the cell
Background image of page 2
3 ht p:/ www.zoology.ubc.ca/~berger/B200sample/unit_8_protein_pro cessing/vesicle_traf ic/autoradiography.htm Vesicles bud from Golgi and mature into secretory vesicles.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The path 1) New proteins could be traced on rER 2) Move to intermediate vesicles 3) Delivered to the Golgi complex 4) Vesicles bud from Golgi and mature into secretory vesicles [Study Fig 8.4 – Pathway traced with GFP] Protease Newly-made proteins
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/08/2010 for the course BSCI 0103 taught by Professor E.armstong during the Spring '09 term at Maryland.

Page1 / 16

8 - Sorting of newly-made proteins Fall 2009 SF - Sorting...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online