Lecture 2 08-31 - Lecture 2 Methods in Genetic Analysis...

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Lecture 2 Methods in Genetic Analysis
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Outline Tools for genetic analysis DNA isolation and analysis PCR and Sequencing Genomic or cDNA libraries Cloning Probing (clones or isolated DNA) mRNA (expression) Proteins – immunodetection – western blotting
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Agarose electrophoresis of DNA
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Agarose electrophoresis of DNA
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Acrylamide electrophoresis: protein OR single nucleotide resolution
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P olymerase C hain R eaction -- PCR Kary Mullis Nobel Prize Chemistry 1993
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Early PCR-based sequencing technology
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http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=genomes.figgrp.6477 Current approach
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Costs and time for sequencing a human genome (3.2 billion bp) The goal of Helicos is the $1,000 genome in ~ two or three years. That could whole genome sequencing a routine medical procedure. The cost for the Heliscope Single Molecule Sequencer costs roughly $1 million…. Helicos released last year ~50 K per genome
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ariable N umber T andem R epeats Which is the correct suspect? Inidividuals can be identified by unique features of their DNA
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Lecture 2 08-31 - Lecture 2 Methods in Genetic Analysis...

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