1. Ch14_Dec_7_with_Q - 12/8/09 Transposableelements

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12/8/09 1 Transposable elements 1.If the inser±on is autonomous, the kernel will be stable yellow. 2.If the inser±on is autonomous, the kernel will be unstable yellow with purple spots. 3.If the inser±on is nonautonomous, the kernel will be stable yellow. This ques±on relates to the inser±on of a TE into the colour gene of corn. Control of Gene Expression in Eukaryotes DiFerent cell types result from diFerences in gene expression in: DiFerent ±ssues; DiFerent cells of same ±ssue; Same cells/±ssues at diFerent stages of development Gene silencing involves changes in chroma±n structure Nucleosome = 4 types of histones (H2A, H2B, H3, H4) x 2 ~ 200 bp of DNA wrapped around Histone H1 binds DNA b/w nucleosomes
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12/8/09 2 Levels of Transcrip±on. . Basal transcrip±on state promotes high levels of transcrip±on. Changes in chroma±n structure help reduce basal transcrip±on Transcrip±onally inac±ve DNA Is transcribed DNA packaged in (around) nucleosomes ? If so, what changes must occur in nucleosomes during transcrip±on Transcrip±onally Inac±ve DNA Ac±ve DNA For transcrip±on to occur – need remodelling proteins – remove or reposi±on nucleosomes E.g., in yeast – SWI‐SNF mul±subunit complex Transcription
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1. Ch14_Dec_7_with_Q - 12/8/09 Transposableelements

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