2. Ch15_Nov6_ - rep IS3 pro leu thr tra Chromosome Inserted...

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11/6/09 1 DNA is transferred from a living bacterial donor to a living bacterial recipient cell through: 1. Conjuga±on 2. Transforma±on 3. Transduc±on 4. All of the above. 5. Only 2 of the above (A through C).
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11/6/09 2 Gene Transfer in Bacteria Asymmetric gene±c transfer Transfer of the F factor (F plasmid)
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11/6/09 3 Transfer of bacterial chromosomal genes; producAon of recombinants If F plasmid integrates into chromosome, cell is called Hfr (“ h igh f requency of r ecombinaAon”). Hfr cells can transfer chromosomal genes to F cell.
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11/6/09 4 What Horizontal Gene Transfer: Hfr donors There are several places on bacterial chromosome where F plasmid may integrate – di±erent Hfr strains. oriT rep tra F plasmid Recombination lac Chromosome pro pro IS 3 IS 3 IS 3 rep oriT tra IS 3 lac See Figure 15.13
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11/6/09 5 Transfer to F recipient oriT
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Unformatted text preview: rep IS3 pro leu thr tra Chromosome Inserted F plasmid lac IS3 gal trp his arg Horizontal Gene Transfer: Hfr donors 11/6/09 6 RecombinaAon Redfeld, R. J. 2001. Do bacteria have sex? Nature Rev. Gen. 2 : 634‐639. 11/6/09 7 Plaques Bacteria (in lawn - not isolated colonies, covering most of plate) • Bacteriophages- viruses that infect bacteria. • Plaque- area of clearing in bacterial lawn caused by growth inhibition or lysis of bacteria. TransducAon & Transfer of DN± • Movement of bacterial DN± from 1 bacterial cell to another • Transferred via bacteriophage (transferred inside head of phage) 11/6/09 8 Natural TransformaAon ±symmetric geneAc transfer Free DN± taken up from environment DN± transfer from live donor cell in contact with recipient DN± transfer from donor cell to recipient by phage (virus)...
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This note was uploaded on 01/10/2010 for the course BIOLOGY biol2040 taught by Professor Tamarakelly during the Fall '09 term at York University.

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2. Ch15_Nov6_ - rep IS3 pro leu thr tra Chromosome Inserted...

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