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4. Ch5_Oct_23_posted - LinkageMaps y 0 w 1.1 1.1 32.1 v...

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10/25/09 1 y w v m r 1.1 32.1 4 17.8 0 1.1 33.2 37.2 55 NB: In any given cross, recombina4on frac4on can’t be >50% (=unlinked) Gene4c maps can have >50 m.u. b/c cumula4ve across a chromosome. Linkage Maps Describing Linkage Cis arrangement: mutant alleles are on same homologue Trans arrangement: mutant alleles are on different homologues + + A B Cis heterozygote Trans heterozygote Oct 23 rd
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10/25/09 2 Genes on the X chromosome can be mapped without a testcross. 1. True 2. False Two Genes 2 point cross = cross with 2 loci 2 point testcross = cross b/w dihybrid parent & homozygous recessive individual Determining Gene Loca4on Three Genes 3 point testcross (3 point crosses too) ‐ advantages over 2 point crosses Can establish rela4ve order of all 3 genes – beXer image Can analyze effects of mul4ple X.O. events on map distances Represen-ng Genes 1. bb prpr cc 2. b/b; pr/pr; c/c 3. b pr c/b pr c
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10/25/09 3 Test individual (here F 1 female) has to be heterozygous for the genes of interest.
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