7. Ch14_Dec_4_postlecture

7. Ch14_Dec_4_postlecture - A gene that causes chromosome...

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12/6/09 1 All you wanted to know about transposable elements… A gene that causes chromosome breakage 130 Identification of broken chromosomes: effects on phenotype Transposable elements with a dele>on in the coding region for transposase (enzyme helps cut out TE & insert TE into new loca>on) cannot move at all within the genome. 1. True 2. False
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12/6/09 2 They’re everywhere!! Found in virtually all organisms, from bacteria to humans Many different types (families) of elements with different origins & mechanisms of replication Present in very high abundance in some spp e.g., human genomes are composed of almost 50% TEs 132 2 major classes (according to transposition events): 1. Transposons 2. Retrotransposons a) SINEs b) LINEs ~ 7% of human genome!! (~ 3 000 000 kb) 2 Main Types of TEs 1. DNA transposons Replicate via DNA Cut & paste 133 Transposon structure
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12/6/09 3 TEs are self‐replica>ng genes Autonomous Non-autonomous Non-autonomous TEs can use the transposase produced by a related autonomous TE Corn & Transposable Elements 135
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7. Ch14_Dec_4_postlecture - A gene that causes chromosome...

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