3-NAD - Problem Set III, Oxidation/Reduction 1. A...

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Problem Set III, Oxidation/Reduction 1. A Washington researcher, distracted by dreams of the Rose Bowl, proposes to increase the yield of energy from glycolysis by engineering cells to reduce pyruvate to lactate with Washox, a new compound that has an E ° ' of -0.64. a. The E ° ' of pyruvate + 2H + + 2e - is -0.19V. With F = 23 kcal/volt/mol, discuss arguments for and against the researcher's prediction that this particular reaction could be used to generate an additional ATP. b. What other factors would you need to worry about before attempting to engineer the fermenting cell to use Washox for all applicable reactions in glycolysis. Explain. (Qualitative answers are sufficient. Do not worry about other pathways.) 2. A biochemist decides to “improve” an organism that ferments glucose to lactate by deleting the step that converts pyruvate to lactate. He reasons that the new organism would be saving the energy that would otherwise have gone into producing the enzyme for that reaction. The new organism would excrete the pyruvate directly. Comment on his idea.
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This note was uploaded on 01/12/2010 for the course MCB 100B taught by Professor Alper/calendar during the Fall '08 term at Berkeley.

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3-NAD - Problem Set III, Oxidation/Reduction 1. A...

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