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6-e-trans & ox phos

6-e-trans & ox phos - Problem Set 6 Electron...

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Problem Set 6 Electron Transport/Oxidative Phosphorylation 1. A colleague studying oxidative phosphorylation uses isolated, functioning mitochondria and continuously washes the intermembrane space with neutral buffer. He demonstrates that this effectively neutralizes the effect that the pumping of protons by the electron transport chain has on the pH of the intermembrane space. The mitochondria continue to produce ATP. Thus he concludes that an alternative mechanism to that described by the chemi-osmotic hypothesis must exist. 2. Bacterial flagella function is dependent on the energy available from the proton motive force. What is the proton motive force and how is it created? 3. Certain organisms that grow in the absence of oxygen are known to use the proton motive force to power transport and other energy-requiring processes. a. Describe and explain the two components of the proton motive force. b. How, in aerobic organisms, is the proton motive force produced? Explain why this mechanism is dependent on the presence of oxygen.
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